Temperature-responsive glass coverslips with an ultrathin poly (N-isopropylacrylamide) layer
Temperature-responsive glass coverslips with an ultrathin poly (N-isopropylacrylamide) layer
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DOI：
10.1016/j.actbio.2008.06.018
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发表时间：
2009-01-01
影响因子：
9.7
通讯作者：
Okano, Teruo
中科院分区：
文献类型：
--
作者：
Fukumori, Kazuhiro;Akiyama, Yoshikatsu;Okano, Teruo
通过电子束照射将温度响应性交联聚合物凝胶共价接枝到玻璃盖玻片上。聚合物的接枝厚度和数量以及表面润湿性随着初始单体浓度的增加而增加。当单体浓度为5wt.%时，接枝聚合物密度为0.84μg·cm(-2)，细胞在37℃时在表面粘附和铺展，但在20℃时脱落。相反，当单体浓度为35wt.%时，聚合物密度为1.28μg·cm(-2)，即使在37℃下表面也具有细胞排斥性。 这些结果与从温度响应性聚合物接枝组织培养聚苯乙烯培养皿中获得的结果形成显着对比，因为当这些表面上的接枝密度约为μg cm(-2)时，各种类型的细胞表现出温度依赖性细胞粘附/分离。我们讨论了这种差异背后可能的分子机制。 (C) 2008 Acta Materialia Inc. 由 Elsevier Ltd 出版。保留所有权利。
A temperature-responsive cross-linked polymer gel was covalently grafted onto glass coverslips by electron beam irradiation. The grafted thickness and amount of polymer as well as the surface wettability increased with the initial monomer concentration. When the monomer concentration was 5 wt.%, the grafted polymer density was 0.84 mu g cm(-2), and cells adhered and spread on the surface at 37 degrees C, but detached at 20 degrees C. In contrast, when the monomer concentration was 35 wt.%, the polymer density was 1.28 mu g cm(-2), and the surfaces were cell repellent even at 37 degrees C. These results show a remarkable contrast to those obtained from temperature-responsive polymer-grafted tissue culture polystyrene dishes, since various types of cells showed temperature-dependent cell adhesion/detachment when the grafted density was around mu g cm(-2) on these surfaces. We discuss the possible molecular mechanisms underlying this discrepancy. (C) 2008 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.