Development of sorting, aligning, and orienting motile sperm using microfluidic device operated by hydrostatic pressure
Development of sorting, aligning, and orienting motile sperm using microfluidic device operated by hydrostatic pressure
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DOI：
10.1007/s10404-006-0142-3
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发表时间：
2007-10-01
影响因子：
2.8
通讯作者：
Critser, John K.
中科院分区：
文献类型：
--
作者：
Seo, Duck-bong;Agca, Yuksel;Critser, John K.
体外受精(IVF)和卵胞浆内单精子注射(ICSI)是克服男性不育问题最常用的辅助生殖技术。IVF和ICSI程序的障碍之一是从任何给定的精子样本中分离活动精子和非活动精子，以选择最有能力的精子群体。此外，头部和尾部的定向和分离是ICSI的另一个障碍。利用精子沿流动方向的自身运动，可以用廉价的聚合物微流控系统分离运动和非运动的精子。在本文中，我们描述了一种通过低成本制造工艺获得的微流控系统的开发。我们报告了三种不同物种：公牛、小鼠和人的静水压力精液分选的实验结果。在显微镜下观察细胞在这些通道中的运动，并用数码相机记录下来。结果表明，利用运动精子的静水压力和自身运动可以解决精子在微通道中的分离、对准和定向问题。
In vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) are the most commonly used assisted reproductive technologies to overcome male infertility problems. One of the obstacles of IVF and ICSI procedures is separating motile sperm from non-motile sperm to select the most competent sperm population from any given sperm sample. In addition, orientation and separation of the head from the tail is another obstacle for ICSI. Using the self-movement of sperm against flow direction, motile and non-motile sperm can be separated with an inexpensive polymeric microfluidic system. In this paper, we describe the development of a microfluidic system obtained through low-cost fabrication processes. We report experimental results of sperm sorting using hydrostatic pressure of three different species: bull, mouse, and human. The movement of cells in these channels was observed under a microscope and recorded with a digital camera. It is shown that the hydrostatic pressure and self-movement of motile sperm can be used to solve separating, aligning and orienting sperm in the microchannel.