Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana
Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana
复制标题
DOI：
10.1016/j.ydbio.2009.03.002
复制
发表时间：
2009-05-15
影响因子：
2.7
通讯作者：
Umeda, Masaaki
中科院分区：
文献类型：
--
作者：
Adachi, Sumiko;Nobusawa, Takashi;Umeda, Masaaki
A型细胞周期蛋白依赖性激酶（CDKA）是酵母Cdc 2/Cdc 28 p的直系同源物，被认为在植物生长和器官发生中具有重要功能。以前的研究表明，其激酶活性是由翻译后修饰，如结合细胞周期蛋白和磷酸化，但其转录调控知之甚少。在这里，我们产生了一个启动子解剖系列的拟南芥（拟南芥）CDKA;I，并使用β-葡萄糖醛酸酶（GUS）基因融合的报告构建体在植物中的表达分析。结果表明，芽中存在两种类型的转录调控：一般的数量调控和细胞类型特异性调控。我们确定了一个启动子区域，促进CDKA;1表达在叶表皮，但不是在L1层的拍摄顶端分生组织。该区域也指导远轴侧偏表达，这可能与近轴/远轴侧特化有关。另一个报告构建体表明，CDKA; I在叶片内层的表达是由启动子中的一个独特的调控区控制的。这些结果表明，CDKA; I的转录调控可能通过协调细胞分裂和不同细胞类型的分化在叶的正常发育中发挥关键作用。(C)2009 Elsevier Inc. All rights reserved.
A-type cyclin-dependent kinase (CDKA) is an ortholog of yeast Cdc2/Cdc28p, and is assumed to have an essential function in plant growth and organogenesis. Previous studies revealed that its kinase activity is controlled by post-translational modifications, such as binding to cyclins and phosphorylations, but its transcriptional regulation is poorly understood. Here, we generated a promoter dissection series of Arabidopsis (Arabidopsis thaliana) CDKA;I, and used beta-glucuronidase (GUS) gene-fused reporter constructs for expression analyses in planta. The results revealed two types of transcriptional control in shoots: general quantitative regulation and cell type-specific regulation. We identified a promoter region that promotes CDKA;1 expression in the leaf epidermis, but not in the L1 layer of the shoot apical meristem. This region also directed abaxial side-biased expression, which may be linked to the adaxial/abaxial side specification. Another reporter construct showed that CDKA; I expression in the inner layers of leaves is controlled by a distinct regulatory region in the promoter. These results suggest that the transcriptional regulation of CDKA; I may play a key role in proper development of leaves by coordinating cell division and differentiation of different cell types. (C) 2009 Elsevier Inc. All rights reserved.