Comparative Analysis of Primer-Probe Sets for RT-qPCR of COVID-19 Causative Virus (SARS-CoV-2)
Comparative Analysis of Primer-Probe Sets for RT-qPCR of COVID-19 Causative Virus (SARS-CoV-2)
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DOI：
10.1021/acsinfecdis.0c00464
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发表时间：
2020-09-11
影响因子：
5.3
通讯作者：
Kim, Hong Gi
中科院分区：
文献类型：
--
作者：
Jung, Yujin;Park, Gun-Soo;Kim, Hong Gi
冠状病毒病2019 （COVID-19）是由严重急性呼吸综合征冠状病毒2 （SARS-CoV-2，以前也称为2019- ncov）引起的一种新出现的人类传染病。疫情爆发后的8个月内，全球已确诊超过1000万例COVID-19病例。由于人与人之间的传播很容易发生，而且人间感染率正在迅速上升，因此敏感和早期诊断对于防止全球暴发至关重要。最近，世界卫生组织（世卫组织）公布了由中国疾病预防控制中心（China CDC, China）、Charite '（德国）、香港大学（HKU, Hong Kong）、日本国立传染病研究所（Japan NIID, Japan）、泰国国立卫生研究院（Thailand NIH, Thailand）和美国疾控中心（USA）等不同机构开发的SARS-CoV-2引物探针。在本研究中，我们比较了7组N基因引物探针和3组Orf1基因引物探针对SARS-CoV-2 RNA的检测能力。结果显示，来自日本NIID的“NIID_2019-nCOV_N”和来自中国疾控中心的“ORF1ab”在SARS-CoV-2分子诊断中表现良好，对hCoV-229E、hCoV-OC43和MERS-CoV RNA无非特异性扩增和交叉反应性。因此，应选择合适的NIID_2019-nCOV_N（日本NIID）试剂盒与ORF1ab（中国CDC）试剂盒组合进行灵敏、可靠的SARS-CoV-2分子诊断。
Coronavirus disease 2019 (COVID-19) is a newly emerging human infectious disease caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2, also previously known as 2019-nCoV). Within 8 months of the outbreak, more than 10,000,000 cases of COVID-19 have been confirmed worldwide. Since human-to-human transmission occurs easily and the rate of human infection is rapidly increasing, sensitive and early diagnosis is essential to prevent a global outbreak. Recently, the World Health Organization (WHO) announced various primer-probe sets for SARS-CoV-2 developed at different institutions: China Center for Disease Control and Prevention (China CDC, China), Charite '(Germany), The University of Hong Kong (HKU, Hong Kong), National Institute of Infectious Diseases in Japan (Japan NIID, Japan), National Institute of Health in Thailand (Thailand NIH, Thailand), and US CDC (USA). In this study, we compared the ability to detect SARS-CoV-2 RNA among seven primer-probe sets for the N gene and three primer-probe sets for the Orf1 gene. The results revealed that "NIID_2019-nCOV_N" from the Japan NIID and "ORF1ab" from China CDC represent a recommendable performance of RT-qPCR analysis for SARS-CoV-2 molecular diagnostics without nonspecific amplification and cross-reactivity for hCoV-229E, hCoV-OC43, and MERS-CoV RNA. Therefore, the appropriate combination of NIID_2019-nCOV_N (Japan NIID) and ORF1ab (China CDC) sets should be selected for sensitive and reliable SARS-CoV-2 molecular diagnostics.