Primary and secondary motoneurons use different calcium channel types to control escape and swimming behaviors in zebrafish.
Primary and secondary motoneurons use different calcium channel types to control escape and swimming behaviors in zebrafish.
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初级和次级运动神经元使用不同的钙通道类型来控制斑马鱼的逃逸和游泳行为。
DOI：
10.1073/pnas.2015866117
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发表时间：
2020
影响因子：
11.1
通讯作者：
Brehm,Paul
中科院分区：
文献类型：
--
作者：
Wen,Hua;Eckenstein,Kazumi;Weihrauch,Vivien;Stigloher,Christian;Brehm,Paul
斑马鱼的逃避反应和节律性游泳是由两种功能不同的运动神经元（Mn）介导的两种不同的行为。初级（1°Mn）型抑制并具有大的量子含量（Qc）和高的释放概率（Pr）。相反，第二（2°Mn）型促进并具有低的和可变的Qc和Pr。这种功能的二元性很好地匹配了不同的相关行为，1°Mn提供了强的、奇异的C弯曲，启动逃逸，2°Mn提供了较弱的、有节奏的收缩。这些功能上的区别是我们鉴定了1°Mns中介导递质释放的P/Q型钙通道和2°Mns中介导递质释放的N型通道。值得注意的是，尽管有这些功能和行为上的差异，每个肌细胞上的所有15个单独的突触都由1°Mn终扣和至少一个2°Mn终扣共享。这种突触共享的蓝图提供了一种在单个突触后细胞水平上控制两种不同行为的有效方法。
The escape response and rhythmic swimming in zebrafish are distinct behaviors mediated by two functionally distinct motoneuron (Mn) types. The primary (1°Mn) type depresses and has a large quantal content (Qc) and a high release probability (Pr). Conversely, the secondary (2°Mn) type facilitates and has low and variable Qc and Pr. This functional duality matches well the distinct associated behaviors, with the 1°Mn providing the strong, singular C bend initiating escape and the 2°Mn conferring weaker, rhythmic contractions. Contributing to these functional distinctions is our identification of P/Q-type calcium channels mediating transmitter release in 1°Mns and N-type channels in 2°Mns. Remarkably, despite these functional and behavioral distinctions, all ∼15 individual synapses on each muscle cell are shared by a 1°Mn bouton and at least one 2°Mn bouton. This blueprint of synaptic sharing provides an efficient way of controlling two different behaviors at the level of a single postsynaptic cell.