Cytotoxic T lymphocytes are critical in the control of infectious bronchitis virus in poultry
Cytotoxic T lymphocytes are critical in the control of infectious bronchitis virus in poultry
复制标题
DOI：
10.1016/s0145-305x(99)00072-5
复制
发表时间：
2000-03-01
影响因子：
2.9
通讯作者：
Seo, SH
中科院分区：
文献类型：
--
作者：
Collisson, EW;Pei, JW;Seo, SH
各种传染性支气管炎病毒（IBV）株会引起鸡的呼吸道、肾脏、肠道和生殖系统疾病，尤其是对刚孵化的小鸡。已经开发了使用病毒感染的抗原呈递细胞（APC）和使用Semliki Forest病毒载体感染的表达单个病毒多肽的APC来鉴定Gray毒株IBV特异性细胞毒性T淋巴细胞（CTL）应答的测定法。结果表明，主要组织相容性复合物限制性CTL对IBV感染的早期控制起重要作用。在肺和肾中观察到的病毒载量的动力学与从感染鸡的脾脏制备的效应细胞的IBV特异性CTL活性水平相关。免疫T细胞在感染前的连续转移证明IBV致敏的CD8（+）、α β T淋巴细胞对雏鸡急性感染具有保护作用。病毒颗粒中的CTI决定簇可定位于刺突蛋白和核衣壳蛋白，但不定位于膜蛋白。核衣壳蛋白的羧基末端含有负责诱导针对IBV N蛋白的CTL应答的表位。接种表达Gray N株羧基端的DNA质粒，诱导了与两个不同IBV株交叉反应的CTL。此外，这种潜在的DNA疫苗导致保护雏鸡免受急性感染。(C)2000爱思唯尔科技有限公司版权所有。
Various strains of infectious bronchitis virus (IBV) cause respiratory, kidney, enteric and reproductive illnesses in chickens, especially in newly hatched chicks. Assays have been developed to identify Gray strain IBV-specific cytotoxic T lymphocyte (CTL) responses using viral infected antigen presenting cells (APC) and using the Semliki Forest virus vector infected APC expressing individual Viral polypeptides. It was shown that major histocompatibility complex restricted CTL are responsible for early control of IBV infection. The kinetics of viral load observed in the lungs and kidneys correlated with the level of IBV-specific CTL activity of effector cells prepared from spleens of infected chicks. Adoptive transfer of immune T cells to chicks prior to infection demonstrated that IBV primed CD8(+), alpha beta T lymphocytes could protect chicks from acute infection. CTI, determinants in the viral particle can be mapped to the spike and nucleocapsid proteins but not to the membrane protein. The carboxyl terminus of the nucleocapsid protein houses an epitope(s) responsible for induction of CTL responses to IBV N protein. Inoculation of DNA plasmid expressing the carboxyl terminus of Gray strain N resulted in induction of CTL that cross-react with two distinct IBV strains. In addition, this potential DNA vaccine resulted in protection of chicks against acute infection. (C) 2000 Elsevier Science Ltd. All rights reserved.