BROWNIAN-MOTION OF LIPID DOMAINS IN ELECTROSTATIC TRAPS IN MONOLAYERS
BROWNIAN-MOTION OF LIPID DOMAINS IN ELECTROSTATIC TRAPS IN MONOLAYERS
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DOI：
10.1021/j100389a022
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发表时间：
1990-12-27
影响因子：
--
通讯作者：
BENVEGNU, DJ
中科院分区：
文献类型：
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作者：
MCCONNELL, HM;RICE, PA;BENVEGNU, DJ
荧光显微镜可用于观察空气-水界面处的脂质单层。 这些单分子膜经常表现出共存的脂质相，具有丰富多样的域形状。 各向同性域的尺寸、形状和形状转变可以从现象学上解释为相间线张力λ和μ-2（相邻域中偶极密度差的平方）之间的竞争。 理论上，畴大小、形状和形状转变都取决于比率λ/mu-2。 在目前的工作中，它表明，mu-2可以确定从分析的脂质结构域的布朗运动的振幅。 通过结合测量的平衡尺寸和形状的域，布朗运动，可以在原则上确定在脂质单层的λ和μ-2。
Fluorescence microscopy can be used to visualize lipid monolayers at the air-water interface. These monolayers frequently show coexisting lipid phases, with a rich variety of domain shapes. The sizes, shapes, and shape transitions of isotropic domains can be accounted for phenomenologically in terms of a competition between an interphase line tension, lambda, and mu-2, the square of the difference in the dipole densities in neighboring domains. Theoretically, domain sizes, shapes, and shape transitions all depend on the ratio lambda/mu-2. In the present work it is shown that mu-2 can be determined from an analysis of the amplitude of Brownian motion of lipid domains. By combining measurements of the equilibrium sizes and shapes of domains, and Brownian motion, one can in principle determine both lambda and mu-2 in lipid monolayers.