Effect of recycling wood ash on microbiological and biochemical properties of soils
Effect of recycling wood ash on microbiological and biochemical properties of soils
复制标题
回收木灰对土壤微生物和生化性质的影响
DOI：
--
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
C. Vischetti
中科院分区：
文献类型：
--
作者：
P. Perucci;E. Monaci;C. Casucci;C. Vischetti
由于越来越多地使用木材作为可持续燃料，木灰的产量正在增加。木灰可以在作物土壤中循环利用，作为营养源和石灰剂，以纠正土壤酸度。然而，木灰对土壤质量的影响知之甚少。在这里，我们研究了添加木灰在5和20吨公顷-1的微生物和生物化学性质的样品从0-30厘米表面的三个中性和两个碱性农业土壤的影响。将样品在25 °C和60%持水量下孵育一年。土壤微生物生物量（微生物生物量C和N），一般微生物活性使用荧光素二乙酸酯水解，碱性磷酸酶，芳基硫酸酯酶，过氧化氢酶和o-二酚氧化酶的活性定期与pH值和电导率一起测定。增加微生物生物量C和荧光素二乙酸酯水解发生在较低的木灰剂量，而微生物生物量C在较高的木灰剂量在整个期间下降，和荧光素二乙酸酯水解后4个月。处理样品的微生物C/N比与对照样品的微生物C/N比存在显著差异，表明微生物群落结构发生了变化。微生物生物量和微生物活性的变化与pH值的增加有关，pH值的增加会诱导细菌的发展，从而损害真菌，电导率，以及木灰添加后的营养水平。碱性磷酸酶和芳基硫酸酯酶活性在20 t ha-1时受到弱抑制，过氧化氢酶活性在中性样品中受到刺激，在碱性样品中受到抑制。在所有处理过的样品中观察到的最高邻二酚氧化酶活性可能表明矿化过程减少。研究结果表明，施用20 t ha-1的土壤处理，仅在某些情况下对土壤生化指标有显著影响，在进一步研究土壤的农业化学和物理功能之前，不建议施用5 t ha-1以上的处理。
Wood ash production is increasing due to the increasing use of wood as a sustainable fuel. Wood ash can be recycled in crop soils as a source of nutrients and as a liming agent to correct soil acidity. However, the effect of wood ash on soil quality is poorly known. Here we studied the effect of addition of wood ash at 5 and 20 t ha -1 on microbial and biochemical properties in samples from the 0-30 cm surface of three neutral and two alkaline agricultural soils. The samples were incubated for one year at 25 °C and 60% water-holding capacity. The soil microbiological biomass (microbial biomass C and N), general microbial activity using fluorescein diacetate hydrolysis, and alkaline phosphatase, arylsulphatase, catalase and o-diphenoloxidase activities were determined periodically together with pH and electrical conductivity. Increases in microbial biomass C and fluorescein diacetate hydrolysis occurred at the lower wood-ash dose, whereas microbial biomass C decreased at the higher wood-ash dose over the entire period, and the fluorescein diacetate hydrolysis after 4 months. The microbial C/N ratios of treated samples were significantly different with respect to that of the control, suggesting changes in the structure of the microbial communities. The changes in microbial biomass and microbial activity were related to the increases in pH, which induce the development of bacteria to the detriment of fungi, to the electrical conductivity, and to the nutrient levels after wood-ash addition. Alkaline phosphatase and arylsulphatase activities were weakly inhibited at 20 t ha -1 , and catalase activity was stimulated in the neutral samples and inhibited in the alkaline samples. The highest o-diphenoloxidase activity observed throughout in all of the treated samples may suggest a decrease in the mineralisation process. Our findings reveal that the soil biochemical parameters investigated are significantly affected only in some cases at 20 t ha -1 , suggesting that a treatment exceeding 5 t ha -1 is not recommended until the agro-chemical and physical functions of the soil are further studied.