Study of guinea pig and human antibodies to toluene diisocyanate.
Study of guinea pig and human antibodies to toluene diisocyanate.
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豚鼠和人类甲苯二异氰酸酯抗体的研究。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
M. Karol
中科院分区：
文献类型：
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作者：
M. Karol
甲苯二异氰酸酯(TDI)抗体此前在致敏豚鼠和产业工人中都有报道。TDI抗体的检测是困难的，因为TDI自身聚合和交联载体蛋白的能力导致了制备满意的半抗原-蛋白质结合抗原的问题。使用TDI的单异氰酸酯类似物来制备抗原消除了这个问题。这项研究比较了3种甲苯基单异氰酸酯异构体和TDI在致敏豚鼠和人类中检测TDI抗体的半抗原能力。利用凝胶扩散技术，来自豚鼠的抗体与邻位、间位和对甲苯基单异氰酸酯半抗原以及TDI半抗原反应。在豚鼠中，TDI半抗原的反应最强，而偏甲苯基异氰酸酯半抗原的反应最弱。另一方面，在TDI致敏的工人中，通过放射过敏吸附试验确定的抗体与每种半抗原反应相似。
Antibodies to toluene diisocyanate (TDI) have previously been reported in both sensitized guinea pigs and industrial workers. Detection of antibodies to TDI is difficult because the ability of TDI to self-polymerize and cross-link carrier proteins causes problems in preparing satisfactory hapten-protein conjugate antigens. Use of monoisocyanate analogues of TDI for antigen preparation eliminates the problem. This study compared 3 tolyl monoisocyanate isomers with TDI for haptenic ability to detect antibodies to TDI in sensitized guinea pigs and humans. Using the gel-diffusion technique, antibodies from guinea pigs reacted with ortho-, meta- and para-tolyl monoisocyanate haptens and with a TDI hapten. In the guinea pigs, reactions appeared to be strongest with the TDI hapten and weakest with meta-tolyl isocyanate hapten. On the other hand, antibodies in TDI-sensitized workers, as determined by radioallergosorbent testing, reacted comparably with each of the haptens.