Chlamydia pneumoniae and osteoporosis-associated bone loss: a new risk factor?
Chlamydia pneumoniae and osteoporosis-associated bone loss: a new risk factor?
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DOI：
10.1007/s00198-012-2217-1
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发表时间：
2013-05-01
影响因子：
4
通讯作者：
Sessa, R.
中科院分区：
文献类型：
--
作者：
Di Pietro, M.;Schiavoni, G.;Sessa, R.
我们发现肺炎衣原体DNA在骨质疏松骨组织和外周血单个核细胞（PBMCs）中的存在与循环再吸收细胞因子的增加有关。该研究包括59名因股骨颈骨折接受髋关节置换手术的妇女：32名患有骨质疏松症，27名患有骨关节炎。共检测118例组织标本（59例骨组织，59例PBMC）的C。pneumoniae DNA，通过实时聚合酶链反应（PCR）。同时测定血清可溶性核因子κ B受体激活因子配体（sRANKL）、骨保护素（OPG）、白细胞介素（IL）-1 β、肿瘤坏死因子-α和IL-6水平。肺炎克雷伯氏菌DNA在骨化骨组织中检测到，而在非骨化骨组织中未检测到（p < 0.05）。C.骨关节炎患者PBMC中pneumoniae DNA阳性率明显高于骨关节炎患者（p < 0.05）。在骨质疏松患者中，血清sRANKL、IL-1和IL-6浓度以及sRANKL/OPG比值在骨组织和PBMC对C阳性的患者之间存在显著差异。pneumoniae和C.肺炎阴性的患者。pneumoniae DNA，骨组织和PBMCs中，sRANKL/OPG比值以及IL-1 β和IL-6水平的增加表明C.肺炎感染作为骨质疏松症的一个新的危险因素。
We found an association between the presence of Chlamydia pneumoniae DNA both in osteoporotic bone tissue and peripheral blood mononuclear cells (PBMCs) and the increase in circulating resorptive cytokines.Our study was designed to determine whether C. pneumoniae infection may be involved in osteoporosis-associated bone loss.The study included 59 women undergoing hip joint replacement surgery for femoral neck fracture: 32 with osteoporosis and 27 with osteoarthritis. A total of 118 tissue specimens (59 bone tissues, 59 PBMCs) were examined for C. pneumoniae DNA by real-time polymerase chain reaction (PCR). Serum levels of soluble receptor activator of nuclear factor kappa B ligand (sRANKL), osteoprotegerin (OPG), interleukin (IL)-1 beta, tumor necrosis factor-alpha, and IL-6 were also measured.C. pneumoniae DNA was detected in osteoporotic bone tissue whereas it was not found in non-osteoporotic bone tissue (p < 0.05). A significantly higher rate of C. pneumoniae DNA (p < 0.05) was found in PBMCs of osteoporotic patients than in those of osteoarthritis patients. Among osteoporotic patients, serum sRANKL, IL-1, and IL-6 concentrations as well as sRANKL/OPG ratio significantly differ between patients with bone tissue and PBMCs positive to C. pneumoniae and C. pneumoniae-negative patients.The association between the presence of C. pneumoniae DNA, both in bone tissue and PBMCs, and the increase in sRANKL/OPG ratio as well as in IL-1 beta and IL-6 levels observed in osteoporotic patients suggests C. pneumoniae infection as a new risk factor for osteoporosis.