GFP chimeric models exhibited a biphasic pattern of mesenchymal cell invasion in tendon healing
GFP chimeric models exhibited a biphasic pattern of mesenchymal cell invasion in tendon healing
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DOI：
10.1002/jcp.20876
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发表时间：
2007-03-01
影响因子：
5.6
通讯作者：
Kubo, Toshikazu
中科院分区：
文献类型：
--
作者：
Kajikawa, Yoshiteru;Morihara, Toru;Kubo, Toshikazu
受损的肌肉骨骼系统的愈合需要大量间充质细胞的涌入，这些细胞可以分化为成骨细胞、成纤维细胞、软骨母细胞和骨骼肌母细胞。然而，这些间充质细胞是来自循环(骨髓)还是来自受损组织本身，一直存在争议。为了揭示修复间质细胞的时空特征，我们利用两种类型的绿色荧光蛋白(GFP)嵌合大鼠，一种在循环细胞中表达GFP，另一种在髌腱中表达GFP，观察了髌腱损伤后的愈合过程。我们分析了两种嵌合大鼠实验性肌腱损伤后GFP阳性细胞的行为，以阐明修复细胞的来源。伤后24小时，创面有循环来源的细胞，但没有肌腱来源的细胞。肌腱来源的细胞在损伤后3d开始出现，随着时间的推移数量显著增加，并在损伤后7d一直保持较高的增殖活性，而循环来源的细胞数量已经减少，并被肌腱来源的细胞所取代。这些发现表明，循环来源的细胞和肌腱来源的细胞在不同时期促进了肌腱的愈合，这是一个双相过程的一部分。
The healing of an injured musculoskeletal system requires an influx of mesenchymal cells that can differentiate into osteoblasts, fibroblasts, chondroblasts, and skeletal myoblasts. However, whether these mesenchymal cells arise from the circulation (bone marrow) or the injured tissues themselves has been controversial. To reveal the spatiotemporal characteristics of the reparative mesenchymal cells, we investigated the healing process after patellar tendon injury using two types of green fluorescent protein (GFP) chimeric rats; one expressing GFP in the circulating cells, and the other expressing it in the patellar tendon. We analyzed the behavior of GFP-positive cells after experimental tendon injury in both chimeric rats to clarify the origin of reparative cells. At 24 h after the injury, the wound contained circulation-derived cells but not tendon-derived cells. Tendon-derived cells first appeared in the wounded area at 3 days after the injury, and had significantly increased in number with time and had maintained a high level of proliferative activity until 7 days after the injury, whereas the circulation-derived cells had decreased in number and had been replaced by the tendon-derived cells. These findings suggest that circulation-derived and tendon-derived cells contribute to the healing of tendons in different periods as part of a biphasic process.