Combined immunohistochemistry for the differential diagnosis of cystic jaw lesions : its practical in surgical pathology
Combined immunohistochemistry for the differential diagnosis of cystic jaw lesions : its practical in surgical pathology
复制标题
联合免疫组织化学鉴别诊断颌骨囊性病变：在外科病理学中的应用
DOI：
10.1111/j.1365-2559.2010.03721.x
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发表时间：
2010
期刊：
影响因子：
6.4
通讯作者：
Saku T
中科院分区：
文献类型：
--
作者：
Tsuneki M;Yamazaki M;Cheng J;Maruyama S;Kobayashi T;Saku T
Souza L R，Fonseca‐Silva T，桑托斯C C O，Oliveira M V M，Corrêa‐Oliveira R，Guimarces A L S & De Paula A M B
（2010）Histology 57，796- 805光化性唇炎和唇鳞状细胞癌中肥大细胞、嗜酸性粒细胞白细胞和微血管密度的关联目的：确定肥大细胞（MC）、嗜酸性粒细胞白细胞（EL）和微血管密度（MVD）在唇癌发生中的贡献，并建立这些生物标志物与其在唇鳞状细胞癌（LSCC）中的可能预后价值之间的关系。方法和结果：将唇粘膜（n=13）、光化性唇炎（n=29）和LSCC（n=29）的存档标本福尔马林固定，石蜡包埋，切片并用甲苯胺蓝和苏木精和曙红（H&E）染色，以鉴定MC和EL并测量它们的密度。通过使用CD 31抗体在染色微血管数量最多的区域（“热点”）测定MVD来估计肿瘤血管生成。在唇肿瘤的发展过程中，MC，EL和MVD的观察进行性增加。相关性分析显示肿瘤进展过程中生物标志物之间存在正相关。在喉鳞状细胞癌样本中，发现MVD值与转移性疾病之间存在显著相关性。在多因素分析中，MVD是宫颈转移风险的预测因子。结论：唇癌发生过程中MC、EL和微血管密度增加，MC和EL可能与刺激肿瘤血管生成有关。MVD可作为预测喉鳞癌颈淋巴结转移的指标。
Souza L R, Fonseca‐Silva T, Santos C C O, Oliveira M V M, Corrêa‐Oliveira R, Guimarães A L S & De Paula A M B
(2010)Histopathology57,796–805Association of mast cell, eosinophil leucocyte and microvessel densities in actinic cheilitis and lip squamous cell carcinomaAims:To determine the contributions of mast cells (MC), eosinophil leucocytes (EL) and microvessel density (MVD) in lip carcinogenesis, and to establish the relationships between these biomarkers and their possible prognostic value in lip squamous cell carcinoma (LSCC).Methods and results:Archived specimens of lip mucosa (n=13), actinic cheilitis (n=29) and LSCC (n=29) were formalin‐fixed, paraffin‐embedded, sectioned and stained with toluidine blue and haematoxylin and eosin (H&E) in order to identify MC and EL and to measure their densities. Tumour angiogenesis was estimated by determining, with the use of CD31 antibody MVD in areas with the highest number of stained microvessels (‘hot spots’). Progressive increases of MC, EL and MVD were observed during lip tumour development. Correlation analysis revealed positive associations between the biomarkers during tumour progression. In LSCC samples, significant associations were found between MVD values and metastatic disease. On multivariate analysis, MVD was a predictor of risk of cervical metastasis.Conclusions:The densities of MC, EL and microvessels increase during lip carcinogenesis, and for MC and EL this may be related to the stimulation of tumour angiogenesis. MVD could be a useful predictor of cervical metastasis in LSCC.