Immuno-enhancement effects of Platycodon grandiflorum extracts in splenocytes and a cyclophosphamide-induced immunosuppressed rat model
Immuno-enhancement effects of Platycodon grandiflorum extracts in splenocytes and a cyclophosphamide-induced immunosuppressed rat model
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DOI：
10.1186/s12906-019-2724-0
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发表时间：
2019-11-21
影响因子：
--
通讯作者：
Lee, Young-Rae
中科院分区：
文献类型：
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作者：
Noh, Eun-Mi;Kim, Jeong-Mi;Lee, Young-Rae
背景桔梗是一种开花植物，传统医药用于治疗肺部和呼吸系统疾病。它具有多种药理作用，包括免疫调节和抗癌活性。本研究旨在证实桔梗提取物(PGE)对环磷酰胺(CP)诱导的免疫抑制大鼠脾细胞的体内外免疫增强作用。方法将不同剂量的PGE与CP共同作用于脾细胞，进行体外分析。用WST-1比色法检测细胞活性，并检测NK细胞活性和细胞毒性T淋巴细胞(CTL)活性。此外，检测免疫球蛋白A(IgA)、免疫球蛋白G和细胞因子水平。为了进行体内分析，SD大鼠在CP的基础上同时给予不同剂量的PGE。进行全血计数(CBC)，测定血浆中IgA、Ig G、肿瘤坏死因子-α、干扰素-γ、白介素2、白介素12水平。此外，通过胸腺和脾的组织学分析来评估组织损伤。结果PGE可提高脾细胞存活率和自然杀伤细胞及细胞毒T淋巴细胞活性，增加CP诱导的炎性细胞因子(肿瘤坏死因子-α、干扰素-γ、白介素2、白介素12)和免疫球蛋白(免疫球蛋白)的产生。此外，在CP治疗的大鼠中，PGE治疗诱导了白细胞、中性粒细胞和淋巴细胞计数的恢复，以及中等绝对值的恢复，并增加了血清中炎性细胞因子(TNF-α、干扰素-γ、IL-2和IL-12)和免疫球蛋白(Ig G和Ig A)的水平。此外，PGE还可减轻CP所致的脾和胸腺损伤。结论PGE在体外和体内均具有免疫增强作用，提示PGE可作为免疫刺激剂的成分或作为功能性食品的成分。
Background Platycodon grandiflorum is a flowering plant that is used in traditional medicine for treating pulmonary and respiratory disorders. It exerts various pharmacological effects, including immunomodulatory and anti-cancer activities. The purpose of this study was to confirm the in vitro and in vivo immune-enhancing effects of P. grandiflorum extract (PGE) on splenocytes isolated from cyclophosphamide (CP)-induced immunosuppressed rats. Methods For in vitro analysis, splenocytes were treated with PGE at various doses along with CP. Cell viability was measured by a WST-1 assay, and NK cell activity and cytotoxic T lymphocyte (CTL) activity was also examined. In addition, immunoglobulin A (IgA), IgG, and cytokine levels were measured. For in vivo analysis, Sprague Dawley rats were treated with various doses of PGE along with CP. Complete blood count (CBC) was performed, and plasma levels of IgA, IgG, TNF-alpha, IFN-gamma, IL-2, and IL-12 were quantified. Additionally, tissue damage was assessed through histological analyses of the thymus and spleen. Results PGE treatment enhanced cell viability and natural killer cell and cytotoxic T lymphocyte activity, and increased the production of CP-induced inflammatory cytokines (TNF-alpha, IFN-gamma, IL-2, and IL-12) and immunoglobulins (IgG and IgA) in splenocytes. In addition, in CP-treated rats, PGE treatment induced the recovery of white blood cell, neutrophil, and lymphocyte counts, along with mid-range absolute counts, and increased the serum levels of inflammatory cytokines (TNF-alpha, IFN-gamma, IL-2, and IL-12) and immunoglobulins (IgG and IgA). Moreover, PGE attenuated CP-induced spleen and thymic damage. Conclusions Our results confirmed that PGE exerts an immune-enhancing effect both in vitro and in vivo, suggesting that PGE may have applications as a component of immunostimulatory agents or as an ingredient in functional foods.