The 16S rRNA gene of Pasteuria penetrans provides an early diagnostic of infection of root-knot nematodes (Meloidogyne spp.)
The 16S rRNA gene of Pasteuria penetrans provides an early diagnostic of infection of root-knot nematodes (Meloidogyne spp.)
复制标题
穿透性巴斯德氏菌 (Pasteuria penetrans) 的 16S rRNA 基因可提供根结线虫 (Meloidogyne spp.) 感染的早期诊断
DOI：
10.1163/156854112x627318
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发表时间：
2012
期刊：
影响因子：
1.2
通讯作者：
K. Davies
中科院分区：
文献类型：
--
作者：
U. Rao;T. Mauchline;K. Davies
寄主和寄生虫之间的分子相互作用是一个活跃的研究领域，根结线虫（植物专性寄生虫）被穿透性巴氏菌寄生为研究先天免疫的各个方面提供了一个模型。使用囊袋系统，我们能够通过PCR证明，在任何显微镜观察寄生前4天感染了隐性长尾丝虫病。袋状系统虽然不是严格的无菌，但提供了一种相对清洁、灵活的方法，与任何土壤系统相比，污染微生物种类的数量大大减少，从而可以操纵和控制线虫感染的早期阶段。
The molecular interactions between hosts and parasites is an active area of research, and the parasitism of root-knot nematodes, obligate parasites of plants, by the hyper-parasitic bacterium Pasteuria penetrans offers a model by which to investigate aspects of innate immunity. Using a pouch system we were able to demonstrate by PCR, infection of Meloidogyne incognita 4 days prior to any microscopic observations of parasitism. The pouch system, although not strictly axenic, offered a relatively clean, flexible approach with a greatly reduced number of contaminating microbial species than in any soil-based system, whereby the early stages of nematode infection could be manipulated and controlled.