Mechanism of stimulation of ornithine decarboxylase activity in transformed mouse fibroblasts.
Mechanism of stimulation of ornithine decarboxylase activity in transformed mouse fibroblasts.
复制标题
转化小鼠成纤维细胞鸟氨酸脱羧酶活性的刺激机制。
DOI：
10.1021/bi00281a037
复制
发表时间：
1983
期刊：
影响因子：
2.9
通讯作者：
Pegg,AE
中科院分区：
文献类型：
--
作者：
Erwin,BG;Seely,JE;Pegg,AE
布拉德利G.作者：James E. Seely，* 和Anthony E. Pegg* 摘要：用血清刺激汇合血清饥饿的SV-3 T3细胞，可使鸟氨酸脱羧酶活性瞬时增强，在12小时内波动至少20倍。对这种涨落产生的机理进行了研究。用放射免疫分析法和α-（二氟甲基）[5- 3 H]鸟氨酸滴定法测定细胞内酶蛋白的含量。用兔抗同种小鼠肾鸟氨酸脱羧酶的特异性抗血清，以α-（二氟甲基）[5- 3 H]鸟氨酸标记的肾鸟氨酸脱羧酶为示踪配体，进行放射免疫分析。酶蛋白量和酶量之间的精确相关性
Bradley G. Erwin, James E. Seely,* and Anthony E. Pegg* abstract: Stimulation of confluentserum-starved SV-3T3 cells by serum produces a transient enhancement of ornithine decarboxylase activity which fluctuatesat least 20-fold over a 12-h period. The mechanism bywhich this fluctuation is produced was investigated. Two techniques for assaying the amount of enzyme protein in these cells were utilized: ra-dioimmunoassay and titration with a-(difluoromethyl)[5-3H] omithine. The radioimmunoassay was carried out by using a specific antiserum prepared in rabbits against homogeneous mouse kidney ornithine decarboxylase and by using a-(difluoromethyl)[5-3H] ornithine-labeled kidney ornithine decarboxylase as the tracer ligand. An exact correlation between the amount of enzyme protein and the amount of enzyme