Tissue engineering using magnetite nanoparticles and magnetic force: Heterotypic layers of cocultured hepatocytes and endothelial cells
Tissue engineering using magnetite nanoparticles and magnetic force: Heterotypic layers of cocultured hepatocytes and endothelial cells
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DOI：
10.1089/1076327041348301
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发表时间：
2004-05-01
影响因子：
--
通讯作者：
Kobayashi, T
中科院分区：
文献类型：
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作者：
Ito, A;Takizawa, Y;Kobayashi, T
建立由各种类型细胞组成的三维，体内类组织的新技术是组织工程的。我们将磁力应用于大鼠肝细胞和人主动脉内皮细胞（HAEC）的异型，分层的共培养系统，该系统不受细胞类型的限制。含有正表电荷的磁铁矿阳离子脂质体，以每个细胞的浓度为38 pg的磁铁矿，改善HAEC中积累的吸附。磁性标记的HAEC在放置磁铁（4000 g）的位置上特异性地积聚在肝细胞单层上，然后粘附以形成一个异型，分层的构造，并紧密接触。与肝细胞的同型培养物或肝细胞和无磁体的HAEC的同型培养物相比，这种共培养的构建体显着增强了肝细胞的白蛋白分泌。这些结果表明，磁铁矿纳米颗粒和磁力的新颖使用，我们称为“基于磁力的组织工程”（MAG-TE），在组织工程方面提供了重大进步。
Novel technologies to establish three-dimensional, in vivo-like tissue consisting of various types of cells are required for tissue engineering. We applied magnetic force to construct a heterotypic, layered coculture system of rat hepatocytes and human aortic endothelial cells (HAECs) that was not limited by cell type. Magnetite cationic liposomes carrying a positive surface charge to improve adsorption accumulated in HAECs at a concentration of 38 pg of magnetite per cell. Magnetically labeled HAECs specifically accumulated onto hepatocyte monolayers at sites where a magnet ( 4000 G) was positioned, and then adhered to form a heterotypic, layered construct with tight and close contact. This cocultured construct significantly ( p < 0.05) enhanced albumin secretion by hepatocytes compared with that in homotypic cultures of hepatocytes or heterotypic cocultures of hepatocytes and HAECs without magnets. These results suggest that this novel use of magnetite nanoparticles and magnetic force, which we refer to as "magnetic force-based tissue engineering" (Mag-TE), offers a major advancement in tissue engineering.