Single cell laser light scattering spectroscopy in a flow cell: repeated sickling of sickle red blood cells.
Single cell laser light scattering spectroscopy in a flow cell: repeated sickling of sickle red blood cells.
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流动池中的单细胞激光散射光谱：镰状红细胞的重复镰状化。
DOI：
10.1016/0167-4889(87)90222-9
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Tanaka,T
中科院分区：
文献类型：
--
作者：
Peetermans,JA;Nishio,I;Ohnishi,ST;Tanaka,T
我们对单个镰刀状红细胞中的血红蛋白聚集体进行了动态激光散射测量。镰刀状红细胞附着在包被多聚L赖氨酸的流动细胞表面。通过反复改变流动溶液，他们被暴露在几个氧合-脱氧循环中。循环的速度被发现是形成不可逆的形态改变和不可逆的血红蛋白聚集体的决定因素。慢循环时，镰刀状红细胞呈不可逆的不规则圆形，20个循环后仍可观察到氧合状态下的血红蛋白聚集。然而，在快速循环中，即使在60个周期之后，这些变化也没有发生。
We performed dynamic laser light scattering measurements of hemoglobin aggregates in single, sickle erythrocytes. Sickle erythrocytes were attached to the poly-(l-lysine)-coated surface of a flow cell. They were exposed to several oxygenation-deoxygenation cycles by repeatedly changing the flowing solution. The rate of cycling was found to be a determining factor for the formation of irreversible morphologic alterations as well as irreversible hemoglobin aggregates. In slow cycling, the sickle erythrocytes took an irreversible, irregular, rounded shape, and hemoglobin aggregates were observed even in the oxygenated state after 20 cycles. In the fast cycling, however, these changes did not take place even after 60 cycles.