Integrated Raman- and angular-scattering microscopy
Integrated Raman- and angular-scattering microscopy
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DOI：
10.1364/ol.33.000714
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发表时间：
2008-04-01
期刊：
影响因子：
3.6
通讯作者：
Berger, Andrew J.
中科院分区：
文献类型：
--
作者：
Smith, Zachary J.;Berger, Andrew J.
已经构建了一种显微镜系统，能够使用宽度小于 10 μm 的单个聚焦激光光斑同时获取传统拉曼光谱和角度分辨弹性散射图案。弹性散射信号通过广义洛伦兹-米氏理论进行分析，我们认为这是该理论对单个球体角反向散射预测的首次实验验证。该显微镜系统在预测球体直径方面具有 3 nm 的精度，同时产生高质量的拉曼信号。设想了单细胞分析的应用。 (C) 2008 年美国光学学会。
A microscopy system has been constructed that is capable of simultaneously acquiring both traditional Raman spectra as well as angle-resolved elastic-scattering patterns using a single focused laser spot less than 10 mu m wide. The elastic-scattering signal was analyzed by generalized Lorenz-Mie theory, representing what we believe to be the first experimental validation of the theory's prediction of angular backscatter from single spheres. The microscope system exhibits 3 nm precision in predicting sphere diameters, while simultaneously yielding high-quality Raman signals. Applications to single cell analysis are envisioned. (C) 2008 Optical Society of America.