CELL AND ORGANELLE SHRINKAGE DURING PREPARATION FOR SCANNING ELECTRON-MICROSCOPY - EFFECTS OF FIXATION, DEHYDRATION AND CRITICAL-POINT DRYING
CELL AND ORGANELLE SHRINKAGE DURING PREPARATION FOR SCANNING ELECTRON-MICROSCOPY - EFFECTS OF FIXATION, DEHYDRATION AND CRITICAL-POINT DRYING
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DOI：
10.1111/j.1365-2818.1977.tb00012.x
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发表时间：
1977-01-01
影响因子：
2
通讯作者：
KIRSCHNER, RH
中科院分区：
文献类型：
--
作者：
GUSNARD, D;KIRSCHNER, RH
临界点干燥法制备样品的扫描电子显微镜[SEM]是与一个变量的试样收缩。在离体小鼠肝细胞核和人红细胞中研究了这种现象的原因。临界点干燥过程本身导致大部分收缩（两个样本的直径减少25-30%）。在临界点干燥之前，戊二醛固定和乙醇脱水仅引起最小的尺寸减小。惰性（乙二醇-乙二醇单乙醚）脱水技术的替代不会改变最终结果。以前的研究使用高分辨率扫描电镜和相关的透射显微镜的孤立的细胞核表明，收缩代表的小型化的细胞器中，所有的结构组件保持其通常的关系。
The critical point drying method of preparing samples for scanning electron microscopy [SEM] is associated with a variable amount of specimen shrinkage. The causes of this phenomenon were studied in isolated mouse hepatocyte nuclei and in human erythrocytes. The critical point drying process itself caused most of the shrinkage (a 25-30% reduction in diameter in both specimens). Glutaraldehyde fixation and ethanol dehydration caused only minimal size reduction, prior to critical point drying. Substitution of an inert (Ethylene glycol-ethylene glycol monethyl ether) dehydration technique did not alter the final result. Previous studies using high resolution SEM and correlative transmission microscopy of isolated nuclei demonstrated that the shrinkage represents a miniaturization of the organelles in which all structural components retain their usual relationships.