Analysis of vesicular trafficking in primary neurons by live imaging.
Analysis of vesicular trafficking in primary neurons by live imaging.
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DOI：
10.1007/978-1-61779-328-8_22
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发表时间：
2011
影响因子：
--
通讯作者：
D. Tampellini;G. Gouras
中科院分区：
文献类型：
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作者：
D. Tampellini;G. Gouras
阿尔茨海默病是最常见的神经退行性疾病，其特征是突触的进行性丧失和大脑中淀粉样蛋白- β (a β)肽的积累。先前的研究表明，在培养的海马切片和小鼠大脑中，突触活动急剧增加（Cirrito等人）。中国生物医学工程学报（英文版）；2005；Kamenetz等人。神经科学进展（英文版）；由于突触活动促进Aβ分泌，它也可能影响其前体淀粉样前体蛋白（APP）的运输和加工。在这里，我们描述了一种方法来研究急性突触激活对树突内APP运输的影响。
Alzheimer’s disease, the most common neurodegenerative disease, is characterized by a progressive loss of synapses and accumulation of amyloid-beta (Aβ) peptides in the brain. Previous studies demonstrated that acute increase in synaptic activity in cultured hippocampal slices and mouse brains (Cirrito et al. Neuron 48: 913–922, 2005; Kamenetz et al. Neuron 37: 925–937, 2003) enhanced secretion of Aβ. Since synaptic activity promotes Aβ secretion, it could also affect the trafficking and processing of its precursor, the amyloid precursor protein (APP). Here, we describe a method to investigate the effect of acute synaptic activation on APP trafficking within dendrites.