PKGIα inhibits the proliferation of cerebral arterial smooth muscle cell induced by oxyhemoglobin after subarachnoid hemorrhage.
PKGIα inhibits the proliferation of cerebral arterial smooth muscle cell induced by oxyhemoglobin after subarachnoid hemorrhage.
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PKGIα抑制蛛网膜下腔出血后氧合血红蛋白诱导的脑动脉平滑肌细胞的增殖。
DOI：
10.1007/978-3-7091-0353-1_29
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
John H. Zhang
中科院分区：
文献类型：
--
作者：
C. Luo;B. Yi;Zhi Chen;Wei;Yujie Chen;Rong Hu;Zhi Liu;H. Feng;John H. Zhang
本研究旨在观察氧合血红蛋白（Oxyhb）诱导的脑动脉平滑肌细胞（CASMC）增殖及腺病毒介导的PKGI（Ad-PKGI）的干预作用，探讨PKGI基因在蛛网膜下腔出血（SAH）后脑血管痉挛（CVS）分子机制中的调控作用。采用组织贴壁法原代培养大鼠CASMCs。采用半定量逆转录聚合酶链反应（RT-PCR）和western blot检测PKG基因转染CASMC后PKGI mRNA和蛋白的表达。MTT法和3 H-TdR掺入法检测细胞增殖情况。Ad-PKGI可转染CASMCS并高效表达。氧合血红蛋白可刺激CASMC增殖，Ad-PKG转染CASMC后3 H-TdR掺入值和MTT吸光度值增加，并可阻断其增殖。提示PKG信号通路可能在SAH后CVS中起重要作用，PKG基因可能成为基因治疗的靶点。
The purpose of the present study was to observe the proliferation of cerebral arterial smooth muscle cell (CASMC) induced by oxyhemoglobin (Oxyhb) and interfered by Adenovirus-mediate-PKGI (Ad-PKGI), and to investigate the potential regulative role of the PKGI gene in the molecule mechanism of cerebral vasospasm (CVS) after Subarachnoid hemorrhage (SAH). Tissue-sticking method was used for primary cultured rat CASMCs. Semi-quantitative reverse transcription and polymerase chain reaction (RT-PCR) and western blot were used to examine the PKGI mRNA and protein expressions after CASMC were transfected by Ad-PKG. The proliferation of CASMCs was determined by MTT assay and 3H-TdR incorporation. Ad-PKGI could be transfected into CASMCS and highly express. Oxyhemoglobin could stimulate the proliferation of CASMC; the value of 3H-TdR incorporation and the absorbance value of MTT increased and could block up after CASMC was transfected by Ad-PKG. The results suggested that the PKG signaling pathway might play an important role in CVS after SAH, and the PKG gene might be a target point of gene therapy.
DOI：
10.1152/ajpheart.00077.2008
发表时间：
2009-07-01
影响因子：
4.8
作者：
Negash, S.;Narasimhan, S. R.;Raj, J. Usha
通讯作者：
Raj, J. Usha
DOI：
10.1161/atvbaha.108.166405
发表时间：
2008-07-01
影响因子：
8.7
作者：
Lukowski, Robert;Weinmeister, Pascal;Feil, Robert
通讯作者：
Feil, Robert