Polylysogeny magnifies competitiveness of a bacterial pathogen in vivo.
Polylysogeny magnifies competitiveness of a bacterial pathogen in vivo.
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DOI：
10.1111/eva.12243
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发表时间：
2015-04
影响因子：
4.1
通讯作者：
Harrison E
中科院分区：
文献类型：
--
作者：
Burns N;James CE;Harrison E
下一代测序的兴起揭示了微生物群落中隐藏的温带噬菌体多样性。虽然其中少数噬菌体已被很好地表征，但对于绝大多数噬菌体携带，特别是多重噬菌体携带的作用，人们知之甚少。利物浦流行的铜绿假单胞菌菌株是囊性纤维化肺部感染的一种侵袭性病原体，最近发现其基因组中含有几种独特的前噬菌体。在这里，我们使用昆虫感染模型实验研究了其中两种噬菌体在体内的作用。我们发现，虽然噬菌体携带在单一细菌感染中没有带来任何好处，但噬菌体通过介导细菌与细菌的竞争在混合感染期间具有巨大的适应性优势。两种噬菌体之间的差异似乎与竞争对手获得噬菌体的速度有关，因此也与耐药性有关。然而，携带两种噬菌体的多溶原的优势最大。这些发现表明，LES 噬菌体可能在宿主入侵中发挥重要作用，更普遍地表明，携带多个噬菌体本身可能是有益的，可以阻碍竞争对手细菌群体中耐药性的传播。
The rise of next generation sequencing is revealing a hidden diversity of temperate phages within the microbial community. While a handful of these phages have been well characterized, for the vast majority, the role of phage carriage, and especially multiple phage carriage, is poorly understood. The Liverpool epidemic strain of Pseudomonas aeruginosa is an aggressive pathogen in cystic fibrosis lung infections that has recently been found to contain several unique prophages within its genome. Here, we experimentally investigate the role of two of these phages in vivo, using an insect model of infection. We find that while no benefit is conferred by phage carriage in single bacterial infections, phages confer a large fitness advantage during mixed infections by mediating bacteria–bacteria competition. Differences between the two phages appeared to be associated with the rate at which the competitor acquired the phage, and therefore resistance. However, the advantage was greatest in the polylysogen, carrying both phages. These findings suggest that the LES phages may play an important role in host invasions and more generally show that the carriage of multiple phages may itself be beneficial by hindering the spread of resistance in rival bacterial populations.
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影响因子：
3
作者：
Fothergill, Joanne L.;Mowat, Eilidh;Winstanley, Craig
通讯作者：
Winstanley, Craig
影响因子：
2.9
作者：
LENSKI, RE;ROSE, MR;TADLER, SC
通讯作者：
TADLER, SC
影响因子：
3.2
作者：
Yasmin, Azra;Kenny, John G.;Horsburgh, Malcolm J.
通讯作者：
Horsburgh, Malcolm J.
影响因子：
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作者：
Figueroa-Bossi, N;Uzzau, S;Bossi, L
通讯作者：
Bossi, L
影响因子：
7
作者：
Winstanley, Craig;Langille, Morgan G. I.;Levesque, Roger C.
通讯作者：
Levesque, Roger C.