Highly resolved in vivo 1H NMR spectroscopy of the mouse brain at 9.4 T
Highly resolved in vivo 1H NMR spectroscopy of the mouse brain at 9.4 T
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DOI：
10.1002/mrm.20184
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发表时间：
2004-09-01
影响因子：
3.3
通讯作者：
Gruetter, R
中科院分区：
文献类型：
--
作者：
Tk치c, I;Henry, PG;Gruetter, R
采用高效的Shim系统和优化的定位序列，在9.4T下测量了C57 BL/6小鼠大脑皮层、海马、纹状体和小脑的活体H-1 NMR谱。自动一阶和二阶匀场（FASTMAP）与强大的定制设计的二阶匀场线圈（匀场强度高达0.04 mT/cm（2））的组合对于实现高光谱分辨率（11-14 Hz的水线宽度）至关重要。评估了在9.4 T下补偿小鼠大脑中的场不均匀性的二阶匀场强度的要求。所获得的光谱质量（分辨率、S/N、水抑制、定位性能）允许从5-10穆尔脑体积可靠地定量16种脑代谢物（LCModel分析）。除Asp、Glc和Gln外，所有定量代谢物均存在显著的区域差异（高达2倍，P < 0.05）。相比之下，从C57 BL/6、CBA和CBA/BL 6小鼠的纹状体测量的H-1 NMR光谱显示，
An efficient shim system and an optimized localization sequence were used to measure in vivo H-1 NMR spectra from cerebral cortex, hippocampus, striatum, and cerebellum of C57BL/6 mice at 9.4 T. The combination of automatic first- and second-order shimming (FASTMAP) with strong custom-designed second-order shim coils (shim strength up to 0.04 mT/cm(2)) was crucial to achieve high spectral resolution (water line width of 11-14 Hz). Requirements for second-order shim strengths to compensate field inhomogeneities in the mouse brain at 9.4 T were assessed. The achieved spectral quality (resolution, S/N, water suppression, localization performance) allowed reliable quantification of 16 brain metabolites (LCModel analysis) from 5-10-muL brain volumes. Significant regional differences (up to 2-fold, P < 0.05) were found for all quantified metabolites but Asp, Glc, and Gln. In contrast, H-1 NMR spectra measured from the striatum of C57BL/6, CBA, and CBA/BL6 mice revealed only small (