Ionizing radiation affects protein composition of exosomes secreted in vitro from head and neck squamous cell carcinoma
Ionizing radiation affects protein composition of exosomes secreted in vitro from head and neck squamous cell carcinoma
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DOI：
10.18388/abp.2015_970
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发表时间：
2015-01-01
影响因子：
1.7
通讯作者：
Pietrowska, Monika
中科院分区：
文献类型：
--
作者：
Jelonek, Karol;Wojakowska, Anna;Pietrowska, Monika
外泌体是参与细胞间通讯的内吞起源的膜囊泡。环境和生理条件影响分泌的外泌体的组成、其丰度以及对受体细胞的潜在影响。在这里，我们分析了暴露于电离辐射（2Gy剂量）的细胞在体外释放的外泌体的蛋白质成分，并将其含量与未受辐射的对照细胞释放的外泌体的成分进行了比较。使用 LC-MS/MS 方法分析源自人鳞状头颈细胞癌的 Fa Du 细胞分泌的外泌体。我们发现暴露于电离辐射会导致外泌体货物发生重大变化。在来自对照细胞的外泌体中鉴定出 217 种蛋白质，在来自辐射细胞的外泌体中鉴定出 384 种蛋白质，其中包括 148 种“常见”蛋白质、236 种在辐射后特异检测到的蛋白质和 69 种在辐射后未检测到的蛋白质。受辐射细胞的外泌体中特别过量表达的蛋白质包括那些参与转录、翻译、蛋白质周转、细胞分裂和细胞信号传导的蛋白质。这表明外泌体货物反映了辐射诱导的细胞过程变化，例如转录和翻译的瞬时抑制或应激诱导的信号传导。
Exosonnes are membrane vesicles of endocytic origin that participate in inter-cellular communication. Environmental and physiological conditions affect composition of secreted exosomes, their abundance and potential influence on recipient cells. Here, we analyzed protein component of exosomes released in vitro from cells exposed to ionizing radiation (2Gy dose) and compared their content with composition of exosomes released from control not irradiated cells. Exosomes secreted from Fa Du cells originating from human squamous head and neck cell carcinoma were analyzed using LC-MS/MS approach. We have found that exposure to ionizing radiation resulted in gross changes in exosomal cargo. There were 217 proteins identified in exosomes from control cells and 384 proteins identified in exosomes from irradiated cells, including 148 "common" proteins, 236 proteins detected specifically after irradiation and 69 proteins not detected after irradiation. Among proteins specifically overrepresented in exosomes from irradiated cells were those involved in transcription, translation, protein turnover, cell division and cell signaling. This indicated that exosomal cargo reflected radiation-induced changes in cellular processes like transient suppression of transcription and translation or stress-induced signaling.