Delayed accumulation of maternal histone mRNA during sea urchin oogenesis.
Delayed accumulation of maternal histone mRNA during sea urchin oogenesis.
复制标题
海胆卵子发生过程中母体组蛋白 mRNA 的延迟积累。
DOI：
10.1016/0012-1606(84)90161-1
复制
发表时间：
1984
影响因子：
2.7
通讯作者：
RafF,RA
中科院分区：
文献类型：
--
作者：
Angerer,LM;DeLeon,DV;Angerer,RC;Showman,RM;Wells,DE;RafF,RA
我们利用原位杂交和RNA印迹技术比较了Poly（A）和α-亚型组蛋白mRNA在海胆卵母细胞发生过程中的积累时间，结果表明，在卵黄发生过程中，卵母细胞中Poly（A）的含量增加了4 ~ 6倍，这意味着多聚腺苷酸化的母体RNA也有类似的增加。相反，RNA印迹和原位杂交表明，在大卵母细胞中几乎没有α亚型组蛋白mRNA。这些结果表明，这些母体mRNA积累在减数分裂成熟完成后的单倍体卵的原核中，它们被储存在那里，直到它们在前期的原核分解期间释放。
We have usedin situhybridization and RNA blotting analysis to compare the timing of accumulation of poly(A) and α-subtype histone mRNA during oogenesis in the sea urchinStrongylocentrotus purpuratus.In situhybridization with3H-poly(U) shows that the content of poly(A) in the developing oocyte increases four- to sixfold during vitellogenesis, implying a similar increase for polyadenylated maternal RNAs. In contrast, both RNA blotting andin situhybridization demonstrate that there is little, if any, α-subtype histone mRNA in large oocytes. These results suggest that these maternal mRNAs accumulate in the pronucleus of the haploid egg after completion of meiotic maturation where they are stored until their release during the breakdown of the pronucleus during prophase.