A photoremovable ligation auxiliary for use in polypeptide synthesis
A photoremovable ligation auxiliary for use in polypeptide synthesis
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DOI：
10.1016/s0040-4039(03)01463-1
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发表时间：
2003-08-04
影响因子：
1.8
通讯作者：
Aimoto, S
中科院分区：
文献类型：
--
作者：
Kawakami, T;Aimoto, S
描述了一种用于通过天然化学连接方法合成多肽的光可移除助剂。2-巯基-1-(2-硝基苯基)乙胺(MnPE-胺)部分通过丝氨酸根的高碘酸氧化作用连接到多肽的N-末端。然后将得到的多肽连接到多肽硫酯上，然后用紫外光(365 Nm)照射，去除多肽中的辅助物。(C)2003爱思唯尔有限公司。保留所有权利。
A photoremovable auxiliary for use in peptide synthesis via the native chemical ligation method is described. A 2-mercapto-1-(2-nitrophenyl)ethylamine (Mnpe-amine) moiety was attached to the N-terminus of a peptide via the periodate oxidation of a seryl peptide. The resulting peptide was then ligated to a peptide thioester, and UV (365 nm) irradiation resulted in the removal of the auxiliary from the peptide. (C) 2003 Elsevier Ltd. All rights reserved.