Analysis of transcripts in methyl jasmonate-treated ginseng hairy roots to identify genes involved in the biosynthesis of ginsenosides and other secondary metabolites
Analysis of transcripts in methyl jasmonate-treated ginseng hairy roots to identify genes involved in the biosynthesis of ginsenosides and other secondary metabolites
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DOI：
10.1007/s00299-004-0845-4
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发表时间：
2005-01-01
影响因子：
6.2
通讯作者：
Liu, JR
中科院分区：
文献类型：
--
作者：
Choi, DW;Jung, JD;Liu, JR
茉莉酸甲酯（MeJA）处理增加了植物次生代谢产物的水平，包括人参皂苷，人参皂苷被认为是人参中的主要活性化合物（Panax ginseng C.A. Meyer）。为了构建包含参皂苷等次生代谢产物生物合成相关基因的人参基因资源，我们从经meja处理的人参毛状根中提取了3134个表达序列标签（ESTs）。这些est组合成370个簇和1,680个单例。产生大量转录本的基因是那些编码脂肪酸去饱和、防御反应和次生代谢物生物合成的蛋白质的基因。对后一组的分析揭示了一些可能参与人参皂苷生物合成的基因，即氧化角鲨烯环化酶（OSC）、细胞色素P450和糖基转移酶。该分析还鉴定出一个新的OSC基因。RNA凝胶印迹分析证实，MeJA处理增加了该OSC基因的转录，以及角鲨烯合成酶（SS）和角鲨烯环氧化酶（SE）基因的转录。该人参EST数据集还将提供有关参与其他次生代谢物生物合成的基因和对MeJA处理有反应的基因的重要信息。
Methyl jasmonate (MeJA) treatment increases the levels of plant secondary metabolites, including ginsenosides, which are considered to be the main active compounds in ginseng (Panax ginseng C.A. Meyer). To create a ginseng gene resource that contains the genes involved in the biosynthesis of secondary metabolites, including ginsenosides, we generated 3,134 expression sequence tags (ESTs) from MeJA-treated ginseng hairy roots. These ESTs assembled into 370 clusters and 1,680 singletons. Genes yielding highly abundant transcripts were those encoding proteins involved in fatty acid desaturation, the defense response, and the biosynthesis of secondary metabolites. Analysis of the latter group revealed a number of genes that may be involved in the biosynthesis of ginsenosides, namely, oxidosqualene cyclase (OSC), cytochrome P450, and glycosyltransferase. A novel OSC gene was also identified by this analysis. RNA gel blot analysis confirmed that transcription of this OSC gene, along with squalene synthase (SS) and squalene epoxidase (SE) gene transcription, is increased by MeJA treatment. This ginseng EST data set will also provide important information on the genes that are involved in the biosynthesis of other secondary metabolites and the genes that are responsive to MeJA treatment.