D2-DOPAMINE RECEPTOR GENE-EXPRESSION BY CHOLINERGIC NEURONS IN THE RAT STRIATUM
D2-DOPAMINE RECEPTOR GENE-EXPRESSION BY CHOLINERGIC NEURONS IN THE RAT STRIATUM
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DOI：
10.1016/0304-3940(90)90671-u
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发表时间：
1990-09-18
影响因子：
2.5
通讯作者：
BLOCH, B
中科院分区：
文献类型：
--
作者：
LEMOINE, C;TISON, F;BLOCH, B
d2受体探针原位杂交和相邻切片胆碱乙酰转移酶（ChAT）抗体免疫组化显示，多巴胺d2受体基因在大鼠尾壳核和伏隔核胆碱能神经元中表达。纹状体中80%的胆碱能神经元含有可检测的d2受体mRNA。与其他胆碱能神经元相比，没有检测到D2mRNA的其他神经元在尾状壳核和伏隔核中没有特定的定位或方面。在某些胆碱能神经元中检测不到D2mRNA可能是由于该程序的灵敏度有限，无法检测到低mRNA水平，或者可以替代地反映纹状体中存在两个胆碱能细胞群，其中一个不表达d2受体基因。其他前脑胆碱能神经元不含D2mRNA。
In situ hybridization with D2receptor probe and immunohistochemistry with choline acetyltransferase (ChAT) antibody performed on adjacent sections demonstrate dopamine D2receptor gene expression in cholinergic neurons of the rat caudate-putamen and nucleus accumbens. Eighty per cent of cholinergic neurons in the striatum contain detectable D2receptor mRNA. The other neurons without detectable D2mRNA do not display specific localization or aspect in the caudate-putamen and nucleus accumbens as compared to the other cholinergic neurons. The absence of detectable D2mRNA in certain cholinergic neurons can be due to the limited sensitivity of the procedure that would not detect low mRNA levels, or alternatively can reflect the existence of two cholinergic cell populations in the striatum, one of which would not express the D2receptor gene. The other forebrain cholinergic neurons do not contain D2mRNA.