Large scale expressed sequence tag (EST) analysis of Metarhizium acridum infecting Locusta migratoria reveals multiple strategies for fungal adaptation to the host cuticle
Large scale expressed sequence tag (EST) analysis of Metarhizium acridum infecting Locusta migratoria reveals multiple strategies for fungal adaptation to the host cuticle
复制标题
对感染飞蝗的绿僵菌的大规模表达序列标签（EST）分析揭示了真菌适应宿主角质层的多种策略
DOI：
10.1007/s00294-012-0382-6
复制
发表时间：
2012-12-01
期刊：
影响因子：
2.5
通讯作者：
Xia, Yuxian
中科院分区：
文献类型：
--
作者：
He, Min;Hu, Jun;Xia, Yuxian
为了感染昆虫宿主，昆虫病原真菌绿僵菌必须突破宿主角质层的物理屏障才能进入昆虫组织。为了筛选毒力相关基因，以提高黑胫病的生防效果。因此，了解M. acridum表达的基因是十分必要的。在其感染昆虫宿主角质层的过程中。在这项研究中，我们进行了大规模的基因表达分析M。蝗虫表皮感染过程中的acridum。我们报告了4，739个独特的表达序列标签（EST）的鉴定，其中80%以前没有被鉴定过。EST分析表明M.蝗属昆虫可能通过代谢重编程、蛋白质分泌、内吞作用、细胞周期调控的形态发生和效应子依赖的免疫抑制等多种适应策略感染蝗虫表皮。通过对病原体-宿主互作数据库的搜索，发现了几个与不同适应过程相关的基因可能编码重要的毒力基因。半定量RT-PCR分析基因表达谱发现，M. acridum差异调节基因的子集，以响应宿主表皮和血淋巴之间的不同感染小生境。本研究为研究M. [医]阿克丁
To infect its insect host, the entomopathogenic fungus Metarhizium acridum has to breach the physical barrier of the host cuticle to gain entry into insect tissue. To identify virulence-associated genes to improve the biocontrol efficacy of M. acridum, it is necessary to understand the genes expressed by M. acridum during its infection of the insect host cuticle. In this study, we performed a large scale gene expression analysis of M. acridum during locust cuticle infection. We report the identification of 4,739 unique expressed sequence tags (ESTs), of which 80 % have not been previously identified. Analysis of the ESTs provided evidence that M. acridum may employ several different adaptation strategies to infect locust cuticle, such as metabolism reprogramming, protein secretion, endocytosis, cell-cycle-regulated morphogenesis and effector-dependent suppression of host immune responses. By searching against the pathogen-host interaction database, several genes associated with different adaptation processes were found to potentially encode important virulence genes. Gene expression pattern analysis by semi-quantitative RT-PCR revealed that M. acridum differentially regulates subsets of genes in response to different infection niches between the host cuticle and hemolymph. The present study provides novel candidate genes to investigate the biology of insect infection by M. acridum.