Apoptosis Signal-Regulating Kinase 1 (ASK1) Activation is Involved in Silver Nanoparticles Induced Apoptosis of A549 Lung Cancer Cell Line
Apoptosis Signal-Regulating Kinase 1 (ASK1) Activation is Involved in Silver Nanoparticles Induced Apoptosis of A549 Lung Cancer Cell Line
复制标题
凋亡信号调节激酶 1 (ASK1) 激活参与银纳米颗粒诱导 A549 肺癌细胞系凋亡
DOI：
10.1166/jbn.2017.2359
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发表时间：
2017-03-01
影响因子：
2.9
通讯作者：
Yu, Dahai
中科院分区：
文献类型：
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作者：
Ma, Jun;Zhao, Di;Yu, Dahai
银纳米粒子（AgNPs）是医学领域最重要的纳米材料之一。我们的研究探讨了AgNPs是否诱导人腺癌A549细胞凋亡及其可能参与凋亡信号调节激酶1（ASK 1）。所制备的银纳米粒子具有较高的稳定性和良好的单分散性。CCK-8法和Hoechst 33342荧光法检测结果显示，它们对A549细胞的增殖具有抑制作用，且呈浓度依赖性。AgNPs显著诱导A549细胞产生活性氧（ROS）。通过免疫细胞化学分析，我们还观察到与未处理的细胞相比，细胞中p-ASK 1的胞质染色增加。此外，我们还发现，经AgNps处理后，ASK 1、c-Jun N末端激酶（JNK）、p38丝裂原活化蛋白激酶（p38）和半胱天冬酶3（caspase 3）被依次激活。因此，银纳米粒子可以抑制肺癌细胞的生长并诱导凋亡。AgNPs的抗癌机制可能与激活ASK 1-JNK/p38-Caspase-3通路有关。
Silver nanoparticles (AgNPs) are one of the most important nanomaterials in the field of medicine. Our study investigated whether AgNPs induce apoptosis of human adenocarcinoma A549 cells and their possible involvement in Apoptosis Signal-Regulating Kinase 1 (ASK1). The prepared AgNPs had high stability and excellent monodispersity. They also possessed inhibitory effect on A549 cells proliferation in a concentration-dependentmanner by Cell Counting Kit-8 (CCK8) assay and Hoechst 33342 Fluorescence. The AgNPs significantly induced production of reactive oxygen species (ROS) in A549 cells. We also observed an increased cytoplasmic staining of p-ASK1 in cells by immunocytochemical analysis, compared with non-treated cells. In addition, we found sequential activation of ASK1, c-Jun N terminal kinase (JNK), p38 Mitogen Activated Protein Kinase (p38) and caspase3 by Western blot after treatment with AgNps. The AgNps may thus inhibit growth of lung cancer cells and induce apoptosis. The anti-cancer mechanism for the AgNPs may be involved in activating the ASK1-JNK/p38-Caspase-3 pathway.