Molecularly imprinted polymer-capped nitrogen-doped graphene quantum dots as a novel chemiluminescence sensor for selective and sensitive determination of doxorubicin
Molecularly imprinted polymer-capped nitrogen-doped graphene quantum dots as a novel chemiluminescence sensor for selective and sensitive determination of doxorubicin
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DOI：
10.1039/c6ra18184h
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发表时间：
2016-01-01
期刊：
影响因子：
3.9
通讯作者：
Jalili, Roghayeh
中科院分区：
文献类型：
--
作者：
Amjadi, Mohammad;Jalili, Roghayeh
本论文采用溶胶-凝胶法制备了分子印迹聚合物封端的氮掺杂石墨烯量子点（MIP@NGQDs），并将其用于抗癌药物阿霉素的识别。当高锰酸钾注入MIP@NGQDs溶液中时，观察到强烈的化学发光（CL）。同时，在多柔比星的存在下，CL强度被显著淬灭，这被用来开发该药物的CL检测方案。通过紫外-可见光谱、荧光光谱和化学发光光谱研究了MIP@NGQDs/KMnO 4化学发光体系的作用机理。在最佳条件下，阿霉素浓度在20-260 mg L-1范围内，相对化学发光强度与阿霉素浓度呈线性关系。检测限为4.7 mg L-1。该方法用于测定加标血清样品中阿霉素的治疗水平，结果令人满意。
In this work, molecularly imprinted polymer-capped nitrogen-doped graphene quantum dots (MIP@NGQDs) were prepared via a simple sol-gel process and used for recognition of the anti-cancer drug, doxorubicin. Strong chemiluminescence (CL) emission was observed when potassium permanganate was injected into the solution of MIP@NGQDs. At the same time, in the presence of doxorubicin the CL intensity was significantly quenched and this was exploited to develop a CL detection scheme for this drug. The mechanism of the MIP@NGQDs/KMnO4 CL system was investigated by recording UV-Vis, fluorescence and CL spectra. Under the optimal conditions, the relative CL intensity linearly decreased with the concentration of doxorubicin in the range of 20-260 mg L-1. The limit of detection was 4.7 mg L-1. The method was applied to the determination of therapeutic levels of doxorubicin in spiked serum samples with satisfactory results.