Cadherin-6 expression transiently delineates specific rhombomeres, other neural tube subdivisions, and neural crest subpopulations in mouse embryos
Cadherin-6 expression transiently delineates specific rhombomeres, other neural tube subdivisions, and neural crest subpopulations in mouse embryos
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DOI：
10.1006/dbio.1996.8501
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发表时间：
1997-03-15
影响因子：
2.7
通讯作者：
Takeichi, M
中科院分区：
文献类型：
--
作者：
Inoue, T;Chisaka, O;Takeichi, M
哺乳动物钙粘蛋白-6（K-cadherin，cad 6）最初是通过聚合酶链反应鉴定的，但其生物学功能尚未确定。我们分析了这种钙粘蛋白的小鼠同源物在发育过程中的表达模式，发现它在限制性菱球以及神经板和神经管的其他亚部中短暂表达。在E8.0-8.5胚胎的中脑和前后脑中，cad 6仅在神经嵴生成区域表达。相反，在这些胚胎的后脑后部和相邻的脊髓中，cad 6出现在整个神经板中，在未来的菱形节4和5的边界处形成了一个尖锐的前界。随后，这种神经板的表达变得局限于菱形节6，虽然大多数的神经嵴生成区域仍然是积极的整个身体。表达cad 6的神经嵴细胞迁移出神经管，随后主要沿沿着聚集。然后，我们研究了Hoxa-1突变对cad 6表达的影响，因为它们的表达在早期后脑后部的时空上相互重叠。在E8.0-8.5 Hoxa-1突变体中，cad 6的表达在菱形4至6的区域被抑制，尽管在其他区域基本上没有受到影响。然而，在晚期，cad 6阳性嵴细胞出现并迁移出第4至第6菱形，表明cad 6表达的抑制是短暂的，仅限于早期阶段。重要的是，Hoxa-1突变的这种效应与该基因表达的时间一致。我们还研究了Hoxa-3突变体，但没有发现这种突变对cad 6表达模式的影响。这些研究结果表明，cad 6可能有助于形成的节段性结构的早期大脑通过其能力，赋予特定的神经细胞和Hoxa-1可能需要早期cad 6的表达在后脑后部。(C)北京：科学出版社.
Mammalian cadherin-6 (K-cadherin, cad6) was originally identified by means of the polymerase chain reaction, but its biological functions have not yet been determined. We analyzed the expression pattern of the mouse homologue of this cadherin during development and found that it was transiently expressed in restricted rhombomeres and in other subdivisions of the neural plate and tube. In the midbrain and anterior hindbrain of E8.0-8.5 embryos, cad6 was expressed only in neural crest-generating regions. In contrast, in the posterior hindbrain and contiguous spinal cord of these embryos, cad6 occurred throughout the neural plate, forming a sharp anterior limit at the future rhombomere 4 and 5 boundary. Subsequently, this neural plate expression became confined to rhombomere 6, although most of the neural crest-generating areas remained positive throughout the body. Neural crest cells expressing cad6 migrated out of the neural tube, and subsequently accumulated mainly along peripheral nerves. We then studied the effect of Hoxa-1 mutation on the expression of cad6, as their expressions spatiotemporally overlapped with each other in the early posterior hindbrain. In E8.0-8.5 Hoxa-1 mutants, cad6 expression was suppressed in the region of rhombomeres 4 to 6, although that in the other regions was not essentially affected. At later stages, however, cad6-positive crest cells appeared and migrated out of rhombomeres 4 to 6, indicating that the suppression of cad6 expression was transient and restricted to early stages. Importantly, this effect of the Hoxa-1 mutation concurred with the timing of the expression of this gene. We also studied Hoxa-3 mutants, but found no effect of this mutation on the cad6 expression pattern. These findings suggest that cad6 may contribute to the formation of the segmental structure of the early brain through its ability to confer specific adhesiveness on cells and that Hoxa-1 may be required for early cad6 expression in the posterior hindbrain. (C) 1997 Academic Press.