Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry for the forensic study of cadaveric volatile organic compounds released in soil by buried decaying pig carcasses
Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry for the forensic study of cadaveric volatile organic compounds released in soil by buried decaying pig carcasses
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DOI：
10.1016/j.chroma.2012.03.048
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发表时间：
2012-09-14
影响因子：
4.1
通讯作者：
Focant, Jean-Francois
中科院分区：
文献类型：
--
作者：
Brasseur, Catherine;Dekeirsschieter, Jessica;Focant, Jean-Francois
本文报道了综合二维气相色谱-飞行时间质谱法（GC x GC- tofms）在法医地理图谱中的应用。在不同深度采集墓葬油样品，分析其挥发性有机化合物（VOC）特征。开发了一种数据处理程序，以突出与分解过程相关的潜在候选标记分子，这些标记分子可以从土壤基质中分离出来。在尸体下面的土壤样本中发现了大约20种特定的化合物，在坟油样本的所有深度都发现了34种其他化合物。这20种化合物包括酮、腈、硫、杂环化合物和苯衍生物，如醛、醇、酮、醚和腈。这34种化合物由甲基支链烷烃异构体组成，包括甲基-、二甲基-、三甲基-、四甲基-和七甲基-异构体，范围从C-12到C-16。观察到这些烷烃在土壤各层中相对存在的趋势，当从胴体到地表时，特定烷烃的数量增加。基于这些甲基支链烷烃在坟墓油中的具体存在，我们创建了一种处理方法，该方法应用特定的脚本来搜索原始数据，以获得与可识别的质量破碎模式相关的特征质谱特征。这种筛选尸体分解特征的土壤样本成功地应用于两个墓地遗址，并清楚地区分了埋葬腐烂猪尸体附近的土壤与对照土壤。(C) 2012 Elsevier B.V.版权所有
This article reports on the use of comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC x GC-TOFMS) for forensic geotaphonomy application. Gravesoil samples were collected at various depths and analyzed for their volatile organic compound (VOC) profile. A data processing procedure was developed to highlight potential candidate marker molecules related to the decomposition process that could be isolated from the soil matrix. Some 20 specific compounds were specifically found in the soil sample taken below the carcass and 34 other compounds were found at all depths of the gravesoil samples. The group of the 20 compounds consisted of ketones, nitriles, sulfurs, heterocyclic compounds, and benzene derivatives like aldehydes, alcohols, ketones, ethers and nitriles. The group of the 34 compounds consisted of methyl-branched alkane isomers including methyl-, dimethyl-, trimethyl-, tetramethyl-, and heptamethyl-isomers ranging from C-12 to C-16. A trend in the relative presence of these alkanes over the various layers of soils was observed, with an increase in the amount of the specific alkanes when coming from the carcass to the surface. Based on the specific presence of these methyl-branched alkanes in gravesoils, we created a processing method that applies a specific script to search raw data for characteristic mass spectral features related to recognizable mass fragmentation pattern. Such screening of soil samples for cadaveric decomposition signature was successfully applied on two gravesoil sites and clearly differentiates soils at proximity of buried decaying pig carcasses from control soils. (C) 2012 Elsevier B.V. All rights reserved.