Effects of neuronal stimulation on mucosal transport in guinea pig ileum.
Effects of neuronal stimulation on mucosal transport in guinea pig ileum.
复制标题
神经元刺激对豚鼠回肠粘膜运输的影响。
DOI：
10.1152/ajpgi.1983.245.2.g290
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Wood,JD
中科院分区：
文献类型：
--
作者：
Cooke,HJ;Shonnard,K;Wood,JD
在豚鼠回肠短路平板上测定了电刺激肠神经对粘膜转运参数的影响。持续时间为0.5 ms的矩形双极刺激脉冲连续施加在与组织平面平行定位的Ag-AgCl箔电极上。电场刺激（EFS）引起的短路电流（Isc）和跨壁电位差的双相增加。对EFS的反应包括Isc的初始快速上升和下降，随后Isc缓慢上升至平台。这种反应持续存在，但有所减弱。Isc的最大变化发生在3.2-5.2 mA的刺激强度和8-10 Hz的频率。刺激诱发的增加Isc可逆地取消了0.1-0.15 μ M河豚毒素，22 mM镁，和0.16 mM镉。EFS之前，9只动物的净钠吸收量为2.3 +/- 0.6 μ eq X cm-2 X h-1，氯化物分泌平均为1.7 +/- 0.8 μ eq X cm-2 X h-1。EFS对单向或净钠通量或组织电导没有影响，而它增加了活性氯分泌从1.7 +/- 0.8至3.9 +/- 0.6 μ eq X cm-2 X h-1。河豚毒素对静息钠或氯通量没有影响。这些结果表明EFS引起豚鼠回肠中氯化物分泌的增加。这些结果表明，EFS通过激活肠神经元改变粘膜转运功能。
The effects of electrical stimulation of enteric nerves on mucosal transport parameters were determined in short-circuited flat sheets of guinea pig ileum. Rectangular bipolar stimulus pulses with a duration of 0.5 ms were applied continuously across Ag-AgCl foil electrodes positioned in parallel with the plane of the tissue. Electrical field stimulation (EFS) elicited a biphasic increase in the short-circuit current (Isc) and transmural electrical potential difference. The response to EFS consisted of an initial rapid rise and decline in Isc followed by a slower rise in Isc to a plateau. This response persisted with some decrement. The maximum change in Isc occurred at stimulus strengths of 3.2-5.2 mA and frequencies of 8-10 Hz. The stimulus-evoked increase in Isc was reversibly abolished by 0.1-0.15 microM tetrodotoxin, 22 mM magnesium, and 0.16 mM cadmium. Prior to EFS, net sodium absorption was 2.3 +/- 0.6 mu eq X cm-2 X h-1 in nine animals, and chloride secretion averaged 1.7 +/- 0.8 mu eq X cm-2 X h-1. EFS had no effect on unidirectional or net sodium fluxes or tissue conductance, whereas it increased active chloride secretion from 1.7 +/- 0.8 to 3.9 +/- 0.6 mu eq X cm-2 X h-1. Tetrodotoxin had no effect on resting sodium or chloride fluxes. These results show that EFS evokes an increase in chloride secretion in guinea pig ileum. These results suggest that EFS alters mucosal transport function by activating enteric neurons.