Simultaneous determination of naproxen, ketoprofen and phenol red in samples from rat intestinal permeability studies: HPLC method development and validation
Simultaneous determination of naproxen, ketoprofen and phenol red in samples from rat intestinal permeability studies: HPLC method development and validation
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DOI：
10.1016/j.jpba.2005.04.008
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发表时间：
2005-09-15
影响因子：
3.4
通讯作者：
Valizadeh, H
中科院分区：
文献类型：
--
作者：
Zakeri-Milani, P;Barzegar-Jalali, M;Valizadeh, H
开发了一种简单的反相高效液相色谱方法，在 270 nm 处进行 UV 检测，用于同时定量酮洛芬和萘普生钠以及酚红作为原位渗透性研究的不可吸收标记物。流动相为20%甲醇、28%乙腈、52%水和0.4ml三乙胺的混合物（用正磷酸调节pH至3.2）。分析以 1.5 ml/min 的流量运行，运行时间为 20 分钟。所有三种化合物的校准曲线在 15.6-250 μg/ml 的浓度范围内均呈线性 (r > 0.999)，萘普生、酮洛芬和酚红的定量限分别为 0.3、0.25 和 0.2 ng/ml。批内和批间精密度变异系数小于或等于5.3%，准确度在95.36%至101.6%之间。使用 SPIP 技术和建议的 HPLC 方法进行样品分析，萘普生和酮洛芬的平均值分别为 1.17e(-4) (+/- 0.28) cm/s 和 0.97e(-4) (+/- 0.2) cm/s。 (c) 2005 Elsevier B.V. 保留所有权利。
A simple reversed-phase high performance liquid chromatographic method with UV detection at 270 nm was developed for simultaneous quantitation of ketoprofen and naproxen sodium along with phenol red as a non-absorbable marker for in situ permeability studies. The mobile phase was a mixture of 20% methanol, 28% of acetonitrile, 52% water and 0.4 ml triethylamine (adjusted to pH 3.2 using orthophosphoric acid). Analysis was run at a flow of 1.5 ml/min with a 20 min run time. The calibration curves were linear for all three compounds (r > 0.999) across the concentration range of 15.6-250 mu g/ml with a limit of quantitation of 0.3, 0.25 and 0.2 ng/ml for naproxen, ketoprofen and phenol red, respectively. The coefficient of variation for intra-assay and inter-assay precision was less than or equal to 5.3% and the accuracy was between 95.36 and 101.6%. Using the SPIP technique and the suggested HPLC method for sample analysis, the mean values of 1.17e(-4) (+/- 0.28) cm/s and 0.97e(-4) (+/- 0.2) cm/s were obtained for naproxen and ketoprofen, respectively. (c) 2005 Elsevier B.V. All rights reserved.