Persister cells and tolerance to antimicrobials
Persister cells and tolerance to antimicrobials
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持久细胞和对抗菌素的耐受性
DOI：
10.1016/s0378-1097(03)00856-5
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发表时间：
2004-01-15
影响因子：
2.1
通讯作者：
Lewis, K
中科院分区：
文献类型：
--
作者：
Keren, I;Kaldalu, N;Lewis, K
细菌种群产生持久性细胞，在杀微生物抗生素的存在下既不生长也不死亡。持久性细菌对抗菌素具有高水平的生物膜耐受性，但人们对它们的生物学几乎一无所知。在不同的生长阶段测试了大肠杆菌对氨苄西林和氧氟沙星的耐受性，以深入了解持久性细菌的性质。在滞后期和指数前期，坚持者的数量没有变化，而在指数中期，坚持者的数量急剧增加。铜绿假单胞菌（氧氟沙星）和金黄色葡萄球菌（环丙沙星和青霉素）也观察到类似的动态。这表明，持久性的产生取决于生长阶段。通过重新接种将大肠杆菌维持在早期指数阶段，以消除持续存在的细菌。这表明，顽固细胞并不处于细胞周期的特定阶段，它们既不是有缺陷的细胞，也不是对抗生素产生反应的细胞。我们的数据表明，持久细胞是特化的存活细胞。(C) 2003年由Elsevier B.V.代表欧洲微生物学会联合会出版。
Bacterial populations produce persister cells that neither grow nor die in the presence of microbicidal antibiotics. Persisters are largely responsible for high levels of biofilm tolerance to antimicrobials, but virtually nothing was known about their biology. Tolerance of Escherichia coli to ampicillin and ofloxacin was tested at different growth stages to gain insight into the nature of persisters. The number of persisters did not change in lag or early exponential phase, and increased dramatically in mid-exponential phase. Similar dynamics were observed with Pseudomonas aeruginosa (ofloxacin) and Staphylococcus aureus (ciprofloxacin and penicillin). This shows that production of persisters depends on growth stage. Maintaining a culture of E coli at early exponential phase by reinoculation eliminated persisters. This suggests that persisters are not at a particular stage in the cell cycle, neither are they defective cells nor cells created in response to antibiotics. Our data indicate that persisters are specialized survivor cells. (C) 2003 Published by Elsevier B.V. on behalf of the Federation of European Microbiological Societies.