Matrix metalloproteinase3 gene promoter polymorphisms and their haplotypes are associated with gastric cancer risk in eastern Indian population
Matrix metalloproteinase3 gene promoter polymorphisms and their haplotypes are associated with gastric cancer risk in eastern Indian population
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DOI：
10.1002/mc.21837
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发表时间：
2012-10-01
影响因子：
4.6
通讯作者：
Swarnakar, Snehasikta
中科院分区：
文献类型：
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作者：
Dey, Sanjib;Stalin, Sami;Swarnakar, Snehasikta
基质金属蛋白酶3（MMP 3）启动子单核苷酸多态性（SNP）在全胃胃癌发生发展中的作用尚未见报道。我们进行了一项以医院为基础的病例对照研究，首次在印度东部人群中探索MMP 3 SNP及其单倍型与胃癌风险的关系。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对218例胃癌患者和175例健康对照者进行MMP 3 -1612 5A/6A（rs3025058）基因分型，并对其中10%进行DNA测序。MMP 3 -707 A/G（rs522616）和MMP 3 -375？通过DNA测序对209例患者和154例对照进行C/G（rs617819）基因分型。MMP 3 -1612 5A 6A基因型（P <0.05）。0.026，比值比（OR）=？1.756，置信区间（CI）？=？1.0702.883），5A 5A和5A 6A联合基因型（P <0.01）。0.015，或？=？1.791，置信区间=？1.1222.858）和5A等位基因（P =？0.002，或？=？1.75，置信区间=？1.212.53）和MMP 3 -707 GG基因型（P <0.01）。
Single nucleotide polymorphisms (SNPs) of matrix metalloproteinase3 (MMP3) promoter in the development and progression of gastric cancer of whole stomach has never been investigated in any population. We conducted a hospital-based case-control study to explore the MMP3 SNPs and their haplotypes with the risk of gastric cancer for the first time in eastern Indian population. A total of 218 gastric cancer patients and 175 healthy controls were genotyped for MMP3-1612 5A/6A (rs3025058) by PCRRFLP and rechecked 10% by DNA sequencing. MMP3-707 A/G (rs522616) and MMP3-375?C/G (rs617819) were genotyped by DNA sequencing among 209 patients and 154 controls. MMP3-1612 5A6A genotype (P?=?0.026, odds ratio (OR)?=?1.756, confidence interval (CI)?=?1.0702.883), combined 5A5A and 5A6A genotype (P?=?0.015, OR?=?1.791, CI?=?1.1222.858) and 5A allele (P?=?0.002, OR?=?1.75, CI?=?1.212.53) and; MMP3-707 GG genotype (P?=?