X-ray structure of the nucleosome core particle.
X-ray structure of the nucleosome core particle.
复制标题
核小体核心颗粒的 X 射线结构。
DOI：
10.1080/07391102.1985.10507623
复制
发表时间：
1985
影响因子：
4.4
通讯作者：
Bunick,GJ
中科院分区：
文献类型：
--
作者：
Uberbacher,EC;Bunick,GJ
本实验室对鸡红细胞核小体的两种单斜晶型（P21,C2）进行了研究。p21晶型的x射线结构已被求解到15 Å分辨率。B-DNA超螺旋具有相对均匀的曲率，在超螺旋中只观察到几个局部扭曲。单个组蛋白结构域已经定位，并且可以观察到每个组蛋白与DNA之间的特定接触。组蛋白与DNA超螺旋内表面的接触主要发生在小凹槽处。大部分组蛋白核心包含在超螺旋DNA的内表面，除了H2A的一部分延伸在DNA末端附近的DNA环流之间。H2A的任何部分都不会阻断DNA末端，也不会阻止DNA顺利进入连接体区域。DNA回圈之间的组蛋白H4部分也有类似的延伸，发生在双轴附近。p21不对称单元中两个独特的核小体表现出良好的双体对称性，并且在整个组蛋白核心和DNA区域彼此相似。
Two monoclinic crystal forms (P21,C2) of chicken erythrocyte nucleosomes have been under study in this laboratory. The x-ray structure of the P21crystal form has been solved to 15 Å resolution. The B-DNA superhelix has a relatively uniform curvature, with only several local distortions observed in the superhelix. The individual histone domains have been localized and specific contacts between each histone and the DNA can be observed. Histone contacts to the inner surface of the DNA superhelix occur predominantly at the minor groove sites. Most of the histone core is contained within the inner surface of the superhelical DNA, except for part of H2A which extends between the DNA gyres near the terminus of the DNA. No part of H2A blocks the DNA terminus or would prevent a smooth exit of the DNA into the linker region. A similar extension of a portion of histone H4 between the DNA gyres occurs close to the dyad axis. Both unique nucleosomes in the P21asymmetric unit demonstrate good dyad symmetry and are similar to each other throughout the histone core and DNA regions.