Differential miRNA expression profiles in bladder urothelial carcinomas.
Differential miRNA expression profiles in bladder urothelial carcinomas.
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发表时间：
2010
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通讯作者：
T. Song;W. Xia;Ningsheng Shao;Xu Zhang;Chun-yang Wang;Yiguang Wu;Jun Dong;W. Cai;
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作者：
T. Song;W. Xia;Ningsheng Shao;Xu Zhang;Chun-yang Wang;Yiguang Wu;Jun Dong;W. Cai;
尿路上皮癌是膀胱肿瘤中最常见的病理类型。创建这种肿瘤类型和正常组织之间差异表达的miRNAs列表可能有助于识别新的诊断和预后标志物。因此，我们对25例膀胱尿路上皮癌和邻近正常膀胱组织进行了本miRNA微阵列分析。结果显示，与正常对照相比，51个差异表达的miRNAs的表达至少有2倍的差异，包括20个上调和31个下调的例子。使用实时RT-PCR分析，实验验证了10种顶级失调miRNA的表达水平，即mir-1、mir-145、mir-143、mir-100、mir-200 b、mir-708、mir-133 a、mir-133 b、mir-125 b和mir-99。这些结果表明，这些miRNAs可能参与膀胱尿路上皮癌的发病机制，并有潜力作为生物标志物。
The urothelial carcinoma is the most common pathological type of bladder tumor. Creation of lists of miRNAs differentially expressed between this tumor type and normal tissue might help identify new diagnostic and prognostic markers. We therefore performed the present miRNA microarray analysis with 25 cases of bladder urothelial carcinomas and adjacent normal bladder tissue. The results showed a panel of 51 differentially expressed miRNAs with at least 2-fold differences in expression compared with the normal controls, including 20 up-regulated and 31 down-regulated examples. The expression levels of ten of the top dysregulated miRNAs, mir-1, mir-145, mir-143, mir-100, mir-200b, mir-708, mir-133a, mir-133b, mir-125b and mir-99 were experimentally verified using real-time RT-PCR analysis. These findings suggest that these miRNAs may be involved in bladder urothelial carcinoma pathogenesis and have potential as biomarkers.