INTERCELLULAR SIGNALING IN VIBRIO-HARVEYI - SEQUENCE AND FUNCTION OF GENES REGULATING EXPRESSION OF LUMINESCENCE
INTERCELLULAR SIGNALING IN VIBRIO-HARVEYI - SEQUENCE AND FUNCTION OF GENES REGULATING EXPRESSION OF LUMINESCENCE
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DOI：
10.1111/j.1365-2958.1993.tb01737.x
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发表时间：
1993-08-01
影响因子：
3.6
通讯作者：
SILVERMAN, MR
中科院分区：
文献类型：
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作者：
BASSLER, BL;WRIGHT, M;SILVERMAN, MR
哈维氏弧菌（Vibrio harveyi）发光的密度依赖型表达受培养基中一种细胞外信号分子（自诱导物）浓度的调控。一个能使一类自发弱光突变体恢复功能的重组克隆被发现编码了合成一种信号分子以及对其作出反应所必需的功能。对编码这些功能的区域进行的序列分析揭示了三个开放阅读框，其中两个（luxL和luxM）是产生一种自诱导物质所必需的，第三个（luxN）是对这种信号物质作出反应所必需的。LuxL和LuxM蛋白在氨基酸序列上与数据库中的其他蛋白不相似，但LuxN蛋白包含的序列区域既类似于组氨酸蛋白激酶，又类似于双组分信号转导蛋白家族的反应调节结构域。具有luxL、luxM和luxN缺陷的突变体的表型表明，一个控制发光的密度依赖型表达的额外信号 - 反应系统仍有待确定。
Density-dependent expression of luminescence in Vibrio harveyi is regulated by the concentration of an extracellular signal molecule (autoinducer) in the culture medium. A recombinant clone that restored function to one class of spontaneous dim mutants was found to encode functions necessary for the synthesis of, and response to, a signal molecule. Sequence analysis of the region encoding these functions revealed three open reading frames, two (luxL and luxM) that are required for production of an autoinducer substance and a third (luxN) that is required for response to this signal substance. The LuxL and LuxM proteins are not similar in amino acid sequence to other proteins in the database, but the LuxN protein contains regions of sequence resembling both the histidine protein kinase and the response regulator domains of the family of two-component, signal transduction proteins. The phenotypes of mutants with luxL, luxM and luxN defects indicated that an additional signal-response system controlling density-dependent expression of luminescence remains to be identified.