AGONIST-INDUCED FLUCTUATIONS IN CYTOPLASMIC CALCIUM IN PRIMARY CULTURES OF BOVINE ENDOTHELIAL-CELLS
AGONIST-INDUCED FLUCTUATIONS IN CYTOPLASMIC CALCIUM IN PRIMARY CULTURES OF BOVINE ENDOTHELIAL-CELLS
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DOI：
10.1113/expphysiol.1991.sp003534
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发表时间：
1991-09-01
影响因子：
2.7
通讯作者：
JOHNSON, C
中科院分区：
文献类型：
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作者：
GILLESPIE, JI;GREENWELL, JR;JOHNSON, C
内皮细胞通过释放局部活性物质在血管对多种刺激的反应中起重要作用。 将外部刺激与活性化合物的释放联系起来的细胞内信号几乎肯定是细胞质钙（Ca（i）2+）的升高。 因此，Ca（i）2+调节的详细知识对于理解内皮细胞的生理学和药理学至关重要。 本实验在单个牛主动脉内皮细胞上进行，证明激动剂刺激Ca（i）2+的复杂变化。 这些包括Ca（i）2+的快速和规则的波动，这与其他内皮细胞和非兴奋细胞中报道的振荡不同。 在含有低钙、2 mM-钴或咖啡因的培养基中，波动完全消除，但如果细胞浸泡在等渗钾溶液中，则不受影响。 提出了Ca（i）2+的波动与钙的局部内流有关，并可能与钙在内库、细胞质和外介质之间的再循环有关的假说。
Endothelial cells play an important role in the vascular responsiveness to many stimuli by releasing locally active agents. The intracellular signal which links the external stimulus to the release of the active compounds is almost certainly an elevation in cytoplasmic calcium (Ca(i)2+). Thus a detailed knowledge of Ca(i)2+ regulation is central to an understanding of the physiology and pharmacology of endothelial cells. The present experiment, on single bovine aortic endothelial cells, demonstrate that agonists stimulate complex changes in Ca(i)2+. These include rapid and regular fluctuations in Ca(i)2+ which are different from the oscillations reported in other endothelial cells and non-excitable cells. The fluctuations are completely abolished in media containing low calcium, 2 mM-cobalt or caffeine but are not affected if the cells are bathed in isotonic potassium solutions. The hypothesis is put forward that the fluctuations in Ca(i)2+ are associated with localized influxes of calcium and are possibly involved with the recycling of calcium between the internal stores, the cytoplasm and the external medium.