Transformation of the White-rot Basidiomycete Coriolus hirsutus Using the Ornithine Carbamoyltransferase Gene
Transformation of the White-rot Basidiomycete Coriolus hirsutus Using the Ornithine Carbamoyltransferase Gene
复制标题
利用鸟氨酸氨基甲酰转移酶基因转化白腐担子菌 Coriolus hirsutus
DOI：
--
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
J. Sugiura
中科院分区：
文献类型：
--
作者：
A. Tsukamoto;Yasushi Kojima;Y. Kita;J. Sugiura
建立了一种适合于担子菌革盖菌的高效转化体系。一个双营养缺陷型突变体。将同时缺乏鸟氨酸氨甲酰基转移酶（OCTase）和3-异丙基苹果酸脱氢酶（3-IPM脱氢酶）的hirsutus用C.新克隆的多毛鱼基因组OCTase基因（arg 1）。转化频率为103-104个转化子/μ gDNA/106-107个原生质体。Southern杂交结果表明，转化DNA以多拷贝形式整合到染色体DNA中。转化子的Arg+表型通过有丝分裂稳定遗传。
An efficient transformation system for the basidiomycete Coriolus hirsutus was developed. A double-auxotrophic mutant of C. hirsutus, deficient both in ornithine carbamoyltransferase (OCTase) and 3-isopropylmalate dehydrogenase (3-IPM dehydrogenase), was transformed to Arg+ with each allelic type of the C. hirsutus genomic OCTase gene (arg1) newly cloned. The transformation frequency of 103-104 transformants per μg DNA per 106-107 oidial protoplasts was reached. Southern blots showed that the transforming DNA was integrated into chromosomal DNA with multi-copies. The Arg+ phenotype of the transformants was stably inherited through mitosis.
使用重组杆状病毒对金孢原毛平革菌活性木质素过氧化物酶进行异源表达和表征。
影响因子：
3.9
作者：
Johnson,TM;Li,JK
通讯作者：
Li,JK