First report of Enterocytozoon bieneusi from dairy cattle in Argentina
First report of Enterocytozoon bieneusi from dairy cattle in Argentina
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DOI：
10.1016/j.vetpar.2013.09.024
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发表时间：
2014-01-17
影响因子：
2.6
通讯作者：
Santin, Monica
中科院分区：
文献类型：
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作者：
Del Coco, Valeria F.;Alejandra Cordoba, Maria;Santin, Monica
粪便样本取自阿根廷布宜诺斯艾利斯 11 个城市的 70 头不到两个月大的奶牛。通过筛分和蔗糖浮选去除粪便碎片后，对样本进行 PCR 检测是否存在比氏肠细胞虫。 PCR 显示 E. bieneusi 的患病率为 14.3%，来自 7 个城市的 10 头小牛呈阳性。对所有 PCR 阳性样本进行基因序列分析，结果显示存在 6 种基因型；四种以前在牛和人类中报道过（D、I、J 和 BEB4），一种以前从未在牛中报道过但以前在人类中报道过（EbpC），以及一种新的基因型（BEB10）。这些结果构成了阿根廷比埃内伊氏菌的首次分子表征，并表明该地区存在人畜共患病传播的潜在风险。 (C) 2013 Elsevier B.V. 保留所有权利。
Fecal specimens were obtained from a total of 70 dairy calves less than two months old on 11 municipalities in Buenos Aires, Argentina. After removal of fecal debris by sieving and sucrose flotation, specimens were subjected to PCR to detect the presence of Enterocytozoon bieneusi. PCR revealed a 14.3% of prevalence for E. bieneusi with 10 positive calves from 7 municipalities. Gene sequence analysis conducted in all samples positives by PCR revealed the presence of six genotypes; four previously reported in cattle as well as humans (D, I, J, and BEB4), one never reported in cattle before but previously reported in humans (EbpC), and one novel genotype (BEB10). These results constitute the first molecular characterization of E. bieneusi in Argentina, and suggest a potential risk of zoonotic transmission in this area. (C) 2013 Elsevier B.V. All rights reserved.