INDEPENDENT HUMAN MAP KINASE SIGNAL-TRANSDUCTION PATHWAYS DEFINED BY MEK AND MKK ISOFORMS
INDEPENDENT HUMAN MAP KINASE SIGNAL-TRANSDUCTION PATHWAYS DEFINED BY MEK AND MKK ISOFORMS
复制标题
DOI：
10.1126/science.7839144
复制
发表时间：
1995-02-03
期刊：
影响因子：
56.9
通讯作者：
DAVIS, RJ
中科院分区：
文献类型：
--
作者：
DERIJARD, B;RAINGEAUD, J;DAVIS, RJ
哺乳动物促分裂原活化蛋白（MAP）激酶包括细胞外信号调节蛋白激酶（ERK）、c-Jun氨基末端激酶（JNK）和p38亚类。这些MAP激酶亚型通过苏氨酸和酪氨酸的双重磷酸化而活化。克隆了两种磷酸化和激活p38 MAP激酶的人MAP激酶激酶（MKK 3和MKK 4）。这些MKK亚型不激活MAP激酶的ERK亚组，但MKK 4激活JNK。这些数据表明，p38（MKK 3和MKK 4），JNK（MKK 4）和ERK（MEK 1和MEK 2）的激活剂定义了独立的MAP激酶信号转导途径。
Mammalian mitogen-activated protein (MAP) kinases include extracellular signal-regulated protein kinase (ERK), c-Jun amino-terminal kinase (JNK), and p38 subgroups. These MAP kinase isoforms are activated by dual phosphorylation on threonine and tyrosine. Two human MAP kinase kinases (MKK3 and MKK4) were cloned that phosphorylate and activate p38 MAP kinase. These MKK isoforms did not activate the ERK subgroup of MAP kinases, but MKK4 did activate JNK. These data demonstrate that the activators of p38 (MKK3 and MKK4), JNK (MKK4), and ERK (MEK1 and MEK2) define independent MAP kinase signal transduction pathways.