Arrow encodes an LDL-receptor-related protein essential for Wingless signalling
Arrow encodes an LDL-receptor-related protein essential for Wingless signalling
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DOI：
10.1038/35035110
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发表时间：
2000-09-28
期刊：
影响因子：
64.8
通讯作者：
DiNardo, S
中科院分区：
文献类型：
--
作者：
Wehrli, M;Dougan, ST;DiNardo, S
Wnt家族的分泌分子在脊椎动物和无脊椎动物发育过程中的细胞命运决定和形态发生中发挥作用（综述见参考文献1）。无翅果蝇是该家族的创始成员，其信号转导级联的许多组分已被鉴定，包括卷曲受体类。但是，无翼信号被接收并通过膜传导的机制还不完全清楚。在这里，我们描述了一个基因，是必要的所有无翅信号事件在果蝇。我们表明，箭头基因的功能是必不可少的细胞接收Wingless输入，它的上游Dishevelled的行为。箭头所示编码的是一种单次跨膜蛋白，表明它可能是卷曲蛋白类受体复合物的一部分。Arrow是一种低密度脂蛋白（LDL）受体相关蛋白（LRP），与鼠和人LRP 5和LRP 6惊人同源。因此，我们的数据表明这个LRP亚家族在Wingless/Wnt信号接收中具有新的和保守的功能。
The Wnt family of secreted molecules functions in cell-fate determination and morphogenesis during development in both vertebrates and invertebrates (reviewed in ref. 1). Drosophila Wingless is a founding member of this family, and many components of its signal transduction cascade have been identified, including the Frizzled class of receptor. But the mechanism by which the Wingless signal is received and transduced across the membrane is not completely understood. Here we describe a gene that is necessary for all Wingless signalling events in Drosophila. We show that arrow gene function is essential in cells receiving Wingless input and that it acts upstream of Dishevelled. arrow encodes a single-pass transmembrane protein, indicating that it may be part of a receptor complex with Frizzled class proteins. Arrow is a low-density lipoprotein (LDL)receptor-related protein (LRP), strikingly homologous to murine and human LRP5 and LRP6. Thus, our data suggests a new and conserved function for this LRP subfamily in Wingless/Wnt signal reception.