The murine testicular transcriptome: Characterizing gene expression in the testis during the progression of spermatogenesis
The murine testicular transcriptome: Characterizing gene expression in the testis during the progression of spermatogenesis
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DOI：
10.1095/biolreprod.103.026880
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发表时间：
2004-07-01
影响因子：
3.6
通讯作者：
Griswold, MD
中科院分区：
文献类型：
--
作者：
Shima, JE;McLean, DJ;Griswold, MD
微阵列最有前途的应用之一是研究与哺乳动物组织生长发育相关的基因表达变化。睾丸提供了一个很好的模型来确定微阵列有效表征器官从出生到成年发育过程中基因表达变化的能力。为此，在Affymetrix MGU74v2 GeneChip平台上创建了一个发育性睾丸基因表达时间过程，分析了11个不同时间点上36000个转录本的表达模式，以更好地了解睾丸发育所必需和引起的分子变化。此外，还建立了分离睾丸细胞类型的基因表达谱，这有助于进一步表征睾丸中每种细胞类型的特定功能行为。对数据进行统计分析，发现11252个转录本（9846个独特转录本）有显著差异表达。随后的聚类分析在时间过程中产生了五种不同的表达模式。这些表达模式在睾丸发育过程中存在不同的时间顺序，并且通常与细胞特异性表达谱有相似之处。对细胞特异性表达模式的分析产生了独特和特征的转录本组，为精子发生过程中睾丸细胞类型的生物学和时间上的活动提供了更深入的了解。对这段时间的进一步分析可以为睾丸的成熟、维持和功能以及精子发生的综合过程中所涉及的已知和未知的基因提供一个明确和更明确的观点。
One of the most promising applications of microarrays is the study of changes in gene expression associated with the growth and development of mammalian tissues. The testis provides an excellent model to determine the ability of microarrays to effectively characterize the changes in gene expression as an organ develops from birth to adulthood. To this end, a developmental testis gene expression time course profiling the expression patterns of similar to36000 transcripts on the Affymetrix MGU74v2 GeneChip platform at 11 distinct time points was created to gain a greater understanding of the molecular changes necessary for and elicited by the development of the testis. Additionally, gene expression profiles of isolated testicular cell types were created that can aid in the further characterization of the specific functional actions of each cell type in the testis. Statistical analysis of the data revealed 11252 transcripts (9846 unique) expressed differentially in a significant manner. Subsequent cluster analysis produced five distinct expressional patterns within the time course. These patterns of expression are present at distinct chronological periods during testis development and often share similarities with cell-specific expression profiles. Analysis of cell-specific expression patterns produced unique and characteristic groups of transcripts that provide greater insight into the activities, biological and chronological, of testicular cell types during the progression of spermatogenesis. Further analysis of this time course can provide a distinct and more definitive view into the genes implicated, known and unknown, in the maturation, maintenance, and function of the testis and the integrated process of spermatogenesis.