LENGTH VARIATION IN THE INTERNAL TRANSCRIBED SPACER OF RIBOSOMAL DNA IN CHANTERELLES
LENGTH VARIATION IN THE INTERNAL TRANSCRIBED SPACER OF RIBOSOMAL DNA IN CHANTERELLES
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DOI：
10.1016/s0953-7562(09)80407-3
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发表时间：
1994-06-01
影响因子：
--
通讯作者：
CIBULA, WG
中科院分区：
文献类型：
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作者：
FEIBELMAN, T;BAYMAN, P;CIBULA, WG
从鸡油菌属（Cantharellus）、壶菌属（Craterellus）和其他被认为与鸡油菌科有亲缘关系的担子菌的31个担子组中提取DNA。利用聚合酶链反应扩增了核核糖体DNA的18 S和28 S基因之间的内转录间隔区（ITS）。除管形鸡油菌和漏斗鸡油菌外，鸡油菌科真菌的ITS区较长，且长度变异较大。18 S和5.8S基因之间的ITS-1区是变异最多的位点。鸡油菌群的ITS-1序列最长，为820 ~ 1100 bp，而其他担子菌的ITS-1序列为240 ~ 350 bp。鸡油菌有最长的和最可变的ITS区尚未描述的真菌。
DNA was extracted from 31 basidiomes of Cantharellus, Craterellus, and other basidiomycetes considered to have affinities to the Cantharellaceae. The internal transcribed spacer (ITS) between the 18S and the 28S genes of the nuclear ribosomal DNA was amplified using the polymerase chain reaction. Except for Cantharellus tubaeformis and Cantharellus infundibuliformis, the ITS region was longer in the Cantharellaceae than in the other fungi tested, and highly variable in length. The ITS-1 region between the 18S and the 5.8S genes was the site of most variation. The Cantharellus cibarius group had the longest ITS-1, ranging from 820 to 1100 base pairs (bp), compared with 240 to 350 bp in other basidiomycetes. Chanterelles have the longest and most variable ITS region yet described for fungi.