Ubiquitination drives COPI priming and Golgi SNARE localization.
Ubiquitination drives COPI priming and Golgi SNARE localization.
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DOI：
10.7554/elife.80911
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发表时间：
2022-07-29
期刊：
影响因子：
7.7
通讯作者：
Graham, Todd R.
中科院分区：
文献类型：
--
作者：
Date, Swapneeta S.;Xu, Peng;Hepowit, Nathaniel L.;Diab, Nicholas S.;Best, Jordan;Xie, Boyang;Du, Jiale;Strieter, Eric R.;Jackson, Lauren P.;MacGurn, Jason A.;Graham, Todd R.
解释高尔基复合体中SNARE定位的机制对于理解分泌途径中的蛋白质运输模式是至关重要的。SNARE也被认为是启动外壳体蛋白I（COPI）组装以确保这些必需货物并入囊泡中，但对这些事件的调控知之甚少。在这里，我们报告的作用泛素识别COPI在陷阱贩运和稳定Arf，COPI，高尔基体陷阱之间的相互作用在酿酒酵母。COPI通过β′-COP的N-末端WD重复结构域或通过不相关的泛素结合结构域结合泛素而不是二赖氨酸基序的能力对于高尔基体SNARE Bet 1和Gos 1的正确定位是必不可少的。我们发现COPI，ArfGAP Glo 3和多个高尔基体SNARE是泛素化的。值得注意的是，Arf和COPI与Gos 1的结合通过这些组分的泛素化而显著增强。Glo 3被提议引发COPI-SNARE相互作用;然而，Glo 3并不富含泛素稳定的SNARE-Arf-COPI复合物，而是富含缺乏SNARE的COPI复合物。这些结果支持了一个新的模型，如何翻译后修饰驱动COPI启动事件的高尔基体陷阱定位至关重要。
Deciphering mechanisms controlling SNARE localization within the Golgi complex is crucial to understanding protein trafficking patterns within the secretory pathway. SNAREs are also thought to prime coatomer protein I (COPI) assembly to ensure incorporation of these essential cargoes into vesicles, but the regulation of these events is poorly understood. Here, we report roles for ubiquitin recognition by COPI in SNARE trafficking and in stabilizing interactions between Arf, COPI, and Golgi SNAREs in Saccharomyces cerevisiae. The ability of COPI to bind ubiquitin, but not the dilysine motif, through its N-terminal WD repeat domain of β′-COP or through an unrelated ubiquitin-binding domain is essential for the proper localization of Golgi SNAREs Bet1 and Gos1. We find that COPI, the ArfGAP Glo3, and multiple Golgi SNAREs are ubiquitinated. Notably, the binding of Arf and COPI to Gos1 is markedly enhanced by ubiquitination of these components. Glo3 is proposed to prime COPI–SNARE interactions; however, Glo3 is not enriched in the ubiquitin-stabilized SNARE–Arf–COPI complex but is instead enriched with COPI complexes that lack SNAREs. These results support a new model for how posttranslational modifications drive COPI priming events crucial for Golgi SNARE localization.
影响因子：
4.6
作者：
Moritsugu K;Nishi H;Inariyama K;Kobayashi M;Kidera A
通讯作者：
Kidera A