Evaluation of selective tumor detection by clinical magnetic resonance imaging using antibody-conjugated superparamagnetic iron oxide
Evaluation of selective tumor detection by clinical magnetic resonance imaging using antibody-conjugated superparamagnetic iron oxide
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DOI：
10.1016/j.jconrel.2012.01.023
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发表时间：
2012-05-10
影响因子：
10.8
通讯作者：
Ishizaka, Yukihito
中科院分区：
文献类型：
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作者：
Koyama, Takayoshi;Shimura, Mari;Ishizaka, Yukihito
单克隆抗体 (mAb) 与纳米级超顺磁性氧化铁 (SPIO) 相结合的主动靶向是磁共振成像 (MRI) 诊断的一种有前景的技术。然而，尚未使用适当的对照来研究该技术的临床适用性。除了高特斯拉实验 MRI 之外，使用临床广泛使用的 1.5 特斯拉 MRI 来评估靶向技术也很重要。在这项研究中，我们使用临床 1.5-Tesla MRI 在体内测量了 mAb 缀合的葡聚糖包被的 SPIO 纳米颗粒 (CMDM)。通过对每只小鼠中来自相同遗传背景的阳性和阴性肿瘤进行简单比较，对荷瘤小鼠进行 MRI。该系统提供了靶肿瘤的显着肿瘤靶向特异性。据我们所知，这是第一份关于使用临床 1.5-Tesla MRI 通过 mAb 偶联 SPIO 特异性检测靶肿瘤的报告。我们的观察结果为在临床应用中使用 mAb 偶联 SPIO 进行可靠的主动靶向提供了线索。 (C) 2012 Elsevier B.V. 保留所有权利。
Active targeting by monoclonal antibodies (mAbs) combined with nanosize superparamagnetic iron oxide (SPIO) is a promising technology for magnetic resonance imaging (MRI) diagnosis. However, the clinical applicability of this technology has not been investigated using appropriate controls. It is important to evaluate the targeting technology using widely used clinical 1.5-Tesla MRI in addition to the high-Tesla experimental MRI. In this study, we measured mAb-conjugated dextran-coated SPIO nanoparticles (CMDM) in vivo using clinical 1.5-Tesla MRI. MRI of tumor-bearing mice was performed using a simple comparison between positive and negative tumors derived from the same genetic background in each mouse. The system provided significant tumor-targeting specificity of the target tumor. To the best of our knowledge, this is the first report on the specific detection of target tumors by mAb-conjugated SPIO using clinical 1.5-Tesla MRI. Our observations provide clues for reliable active targeting using mAb-conjugated SPIO in clinical applications. (C) 2012 Elsevier B.V. All rights reserved.