Molecular and structural assessment of alveolar bone during tooth eruption and function in the miniature pig, sus scrofa.
Molecular and structural assessment of alveolar bone during tooth eruption and function in the miniature pig, sus scrofa.
复制标题
小型猪（sus scrofa）牙齿萌出和功能过程中牙槽骨的分子和结构评估。
DOI：
10.1111/j.1439-0264.2011.01067.x
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Popowics,Tracy
中科院分区：
文献类型：
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作者：
Yeh,Kuang-Dah;Popowics,Tracy
用5张图和2张表格总结牙齿萌出过程中与牙根相邻的牙槽骨的发育情况尚不清楚。这项研究测试了这样的假设：在牙齿萌出期间，主要在牙根附近形成编织骨，但当牙齿发挥功能时，这种不成熟的结构会转变为板层骨。此外，预计骨吸收在将不成熟的骨转变为更成熟的承重组织方面发挥着关键作用。小型猪在两个咬合阶段进行比较，13 周（n= 3），对应于M1牙萌出的粘膜穿透阶段，以及23 周（n= 3），对应于M1/M1早期咬合。用于 RNA 提取和 qRT-PCR 分析的骨样本是从一侧的纵裂和邻近 M1 根处采集的。安乐死后，从另一侧的这些区域采集骨样本进行苏木精和伊红以及 TRAP 染色。与预期相反，萌出的磨牙和功能性磨牙在 M1 附近的牙槽骨中均具有网状纤维板层结构。然而，老年猪的编织骨基质更厚，并且具有更致密的初级骨。基因表达数据和破骨细胞计数显示，磨牙附近的骨吸收活性比远处的骨吸收活性更高，但萌发阶段之间没有差异。因此，虽然吸收确实发生，但它并不是牙槽骨从萌出到功能转变的主要机制。现有编织骨的增量生长和矿化支架内初级骨的填充产生了支持萌出和发挥功能的牙齿所需的强化作用。
With 5 figures and 2 tablesSummaryThe development of alveolar bone adjacent to the tooth root during tooth eruption is not well understood. This study tested the hypothesis that predominantly woven bone forms adjacent to tooth roots during tooth eruption, but that this immature structure transitions to lamellar bone when the tooth comes into function. Additionally, bone resorption was predicted to play a key role in transitioning immature bone to more mature, load‐bearing tissue. Miniature pigs were compared at two occlusal stages, 13 weeks (n= 3), corresponding with the mucosal penetration stage of M1tooth eruption, and 23 weeks (n= 3), corresponding with early occlusion of M1/M1. Bone samples for RNA extraction and qRT‐PCR analysis were harvested from the diastema and adjacent to M1roots on one side. Following euthanasia, bone samples for haematoxylin and eosin and TRAP staining were harvested from these regions on the other side. In contrast to expectations, both erupting and functioning molars had reticular fibrolamellar structure in alveolar bone adjacent to M1. However, the woven bone matrix in older pigs was thicker and had denser primary osteons. Gene expression data and osteoclast cell counts showed a tendency for more bone resorptive activity near the molars than at distant sites, but no differences between eruptive stages. Thus, although resorption does occur, it is not a primary mechanism in the transition in alveolar bone from eruption to function. Incremental growth of existing woven bone and filling in of primary osteons within the mineralized scaffold generated the fortification necessary to support an erupted and functioning tooth.